PXD007802-3

PXD007802 is an original dataset announced via ProteomeXchange.

Title	Maize root LC-MSMS, part 2 - Comparative proteomics of contrasting maize genotypes provides insights into salt-stress tolerance mechanisms
Description	Salt stress is one major abiotic stress limiting maize grain yield throughout the world.To better understand maize salt tolerance molecular mechanism, comparative proteomic analysis was conducted on seedling roots of salt-tolerant genotype Jing724 and salt-sensitive genotype D9H under NaCl. Jing724 exhibited significantly higher germination rate and growth parameters (weight/length) than did D9H under salt treatment.We identified 565 differentially regulated proteins (DRP) using iTRAQ and 89 were specific to Jing724 while 424 were specific to D9H. In salt stressed Jing724, pentose phosphate pathway, glutathione metabolism and nitrogen metabolism were enriched. By pathway enrichment and protein-protein interaction analyses, key DRPs such as glucose-6-phosphate 1-dehydrogenase, NADPH producing dehydrogenase, glutamate synthase and glutamine synthasewere identified.Additionally, salt responsive proteins of Jing724 were indicated to facilitate energy management, maintenance of redox homeostasis, reducing ammonia toxicity, osmotic homeostasis regulation, stress defense, stress adaptation, biotic cross-tolerance and gene transcription regulation. Quantification of multiple metabolic or enzymatic changes including SOD activity, MDA content, relative electrolyte leakage and Proline content were consistent with their predicted changes based on functions of DRPs. DRP analysis was correlated with the mRNA transcripts abundancevariation of eight representative DRPs. These results contribute to elucidating molecular networks of salt tolerance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:20.399.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meijie Luo
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-20 08:43:08	ID requested
1	2017-12-04 23:32:28	announced
2	2017-12-11 04:08:09	announced	Updated publication reference for PubMed record(s): 29192500.
⏵ 3	2024-10-22 04:40:25	announced	2024-10-22: Updated project metadata.

Luo M, Zhao Y, Wang Y, Shi Z, Zhang P, Zhang Y, Song W, Zhao J, Comparative Proteomics of Contrasting Maize Genotypes Provides Insights into Salt-Stress Tolerance Mechanisms. J Proteome Res, 17(1):141-153(2018) [pubmed]

10.1021/acs.jproteome.7b00455;

curator keyword: Biological

submitter keyword: seedling root,iTRAQ, salt tolerance,maize, comparative proteomic analysis

Jiuran Zhao
contact affiliation	Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing, 100097, China
contact email	maizezhao@126.com
lab head
Meijie Luo
contact affiliation	Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing, 100097, China
contact email	mjluo108@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007802

PRIDE project URI

• PRIDE
  1. PXD007802
     1. Label: PRIDE project
     2. Name: Maize root LC-MSMS, part 2 - Comparative proteomics of contrasting maize genotypes provides insights into salt-stress tolerance mechanisms