PXD007800 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cytosolic protein Vms1 links ribosome quality control to mitochondrial and cellular homeostasis |
Description | Eukaryotic cells have evolved extensive protein quality control mechanisms to remove faulty translation products. Here we show that yeast cells continually produce faulty mitochondrial polypeptides that stall on the ribosome during translation but are imported into the mitochondria. The cytosolic protein Vms1, together with the E3 ligase Ltn1, protects against the mitochondrial toxicity of these proteins and maintains cell viability under respiratory conditions. In the absence of these factors, stalled polypeptides aggregate after import and sequester critical mitochondrial chaperone and translation machinery. Aggregation depends on C-terminal alanyl/threonyl sequences (CAT-tails) that are attached to stalled polypeptides on 60S ribosomes by Rqc2. Vms1 binds to 60S ribosomes at the mitochondrial surface and antagonizes Rqc2, thereby facilitating import, impeding aggregation and directing aberrant polypeptides to intra-mitochondrial quality control. Vms1 is a key component of a rescue pathway for ribosome-stalled mitochondrial polypeptides that are inaccessible to ubiquitylation, due to coupling of translation and translocation. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-27 |
AnnouncementXML | Submission_2017-10-27_06:28:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liang Zhao |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-20 07:54:44 | ID requested | |
⏵ 1 | 2017-10-27 06:28:44 | announced | |
2 | 2017-11-08 00:06:33 | announced | Updated publication reference for PubMed record(s): 29107329. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Vms1, Ltn1,Mitochondria, RQC |
Contact List
Walter Neupert |
contact affiliation | 1Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany 2Division of Cell Biology, Biomedical Center, Faculty of Medicine, University of Munich, Großhaderner Str. 9, 82152 Martinsried, Germany |
contact email | neupert@med.uni-muenchen.de |
lab head | |
Liang Zhao |
contact affiliation | Max Planck Institute of Biochemistry |
contact email | zhao@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007800
- Label: PRIDE project
- Name: Cytosolic protein Vms1 links ribosome quality control to mitochondrial and cellular homeostasis