PXD007789-2

PXD007789 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human keratinocytes HaCaT LC-MSMS_SDS exposure
Description	We employed human HaCaT cells as a model system to identify cellular proteins that accompany SDS-induced toxicity based on a proteomic approach. HaCaT human keratinocyte cell line were treated with a non-cytotoxic dose of SDS (25 µg/ml, as determined by the MTT assay and microscopically examination) for 48 h. The altered abundance of proteins from HaCaT keratinocytes exposed to SDS was analyzed by LC-MS/MS approach and quantified using Progenesis LC software. The abundance of 217 proteins (which were identified by multiple peptides, ≥ 2) was altered in keratinocytes exposed to SDS; in which 131 proteins had increased abundance while 86 proteins was down regulated. The Pathview map of 131 up-regulated proteins was built and enhancement of glycolysis/gluconeogenesis was found.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:40.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalia Petushkova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-19 02:31:48	ID requested
1	2017-11-03 03:12:33	announced
⏵ 2	2024-10-22 04:40:47	announced	2024-10-22: Updated project metadata.

Publication List

10.18097/pbmc20176305405;
Rusanov AL, Petushkova NA, Poverennaya EV, Nakhod KV, Larina OV, Lisitsa AV, Luzgina NG, [Proteomic profiling of HaCaT keratinocytes exposed to skin damaging detergents]. Biomed Khim, 63(5):405-412(2017) [pubmed]

10.18097/pbmc20176305405;

Rusanov AL, Petushkova NA, Poverennaya EV, Nakhod KV, Larina OV, Lisitsa AV, Luzgina NG, [Proteomic profiling of HaCaT keratinocytes exposed to skin damaging detergents]. Biomed Khim, 63(5):405-412(2017) [pubmed]

Keyword List

curator keyword: Biological, Biomedical
submitter keyword: HaCaT keratinocytes, shotgun proteomics, sodium dodecyl sulphate, exposure, Pathview map, glycolysis/gluconeogenesis

curator keyword: Biological, Biomedical

submitter keyword: HaCaT keratinocytes, shotgun proteomics, sodium dodecyl sulphate, exposure, Pathview map, glycolysis/gluconeogenesis

Contact List

Andrey V. Lisitsa
contact affiliation	Orekhovich Research Institute of Biomedical Chemistry, Russia
contact email	lisitsa063@gmail.com
lab head
Natalia Petushkova
contact affiliation	Institute of Biomedical Chemistry, Moscow, Russia
contact email	cyp450@mail.ru
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007789
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007789

PRIDE project URI

Repository Record List

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