PXD007787-2

PXD007787 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
Description	The temporal regulation of protein abundance and post-translational modifications is a key feature of cell division. Recently, we analysed gene expression and protein abundance changes during interphase under minimally perturbed conditions (Ly et al. 2014; Ly et al. 2015). Here we show that by using specific intracellular immunolabeling protocols, FACS separation of interphase and mitotic cells, including mitotic subphases, can be combined with proteomic analysis by mass spectrometry. Using this PRIMMUS (PRoteomic analysis of Intracellular iMMUnolabeled cell Subsets) approach, we now compare protein abundance and phosphorylation changes in interphase and mitotic fractions from asynchronously growing human cells. We identify a set of 115 phosphorylation sites increased during G2, which we term 'early risers'. This set includes phosphorylation of S738 on TPX2, which we show is important for TPX2 function and mitotic progression. Further, we use PRIMMUS to provide a proteome-wide analysis of protein abundance remodeling between prophase, prometaphase and anaphase.
HostingRepository	PRIDE
AnnounceDate	2017-10-18
AnnouncementXML	Submission_2019-03-07_05:44:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tony Ly
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-19 02:22:24	ID requested
1	2017-10-18 00:47:49	announced
⏵ 2	2019-03-07 05:44:03	announced	Updated publication reference for PubMed record(s): 29052541.

Ly T, Whigham A, Clarke R, Brenes-Murillo AJ, Estes B, Madhessian D, Lundberg E, Wadsworth P, Lamond AI, Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS. Elife, 6():(2017) [pubmed]

curator keyword: Biological

submitter keyword: Human, Cell Cycle, FACS

Angus Lamond
contact affiliation	Centre for Gene Regulation and Expression University of Dundee
contact email	a.i.lamond@dundee.ac.uk
lab head
Tony Ly
contact affiliation	University of Dundee
contact email	tly@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD007787

PRIDE project URI

• PRIDE
  1. PXD007787
     1. Label: PRIDE project
     2. Name: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS