PXD007779-2

PXD007779 is an original dataset announced via ProteomeXchange.

Title	Effect of acadesine and overexpression of the karyopherin Kap123 on nuclear abundance of yeast proteins
Description	Based on genetic and affinity chromatography experiments, the karyopherin Kap123 was identified as an important player in the cellular response to the drug acadesine (AICAR, 5′-phosphoribosyl-5-amino-4-imidazole carboxamide). Since Kap123 mediates nuclear import of multiple proteins we hypothesized that AICAR could affect nuclear trafficking. The effect of acadesine (AICAR) on nuclear trafficking was assayed by comparing protein composition of enriched nuclear extracts from yeast treated or not with AICAR by label-free quantitative analysis. Total protein extracts from the same cultures were also assayed by quantitative mass spectrometry. A parallel analysis was performed on yeast cells grown in the presence of AICAR and overexpressing or not Kap123 to identify those proteins that are enriched in the nuclear fraction when Kap123 was overexpressed.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:44.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephane Claverol
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-18 05:50:41	ID requested
1	2018-11-23 04:04:09	announced
⏵ 2	2024-10-22 04:40:44	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: karyopherin, yeast, nucleus, AICAR

Benoit Pinson
contact affiliation	University of Bordeaux, IBGC UMR 5095, F-33077 Bordeaux, France
contact email	pinson@ibgc.cnrs.fr
lab head
Stephane Claverol
contact affiliation	Plateforme Proteome
contact email	stephane.claverol@u-bordeaux.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007779
     1. Label: PRIDE project
     2. Name: Effect of acadesine and overexpression of the karyopherin Kap123 on nuclear abundance of yeast proteins