PXD007731-1

PXD007731 is an original dataset announced via ProteomeXchange.

Title	Enhancing Membrane Protein Identification Using a Simplified, Centrifugation and Detergent-based Membrane Extraction Approach
Description	Membrane proteins may act as transporters, receptors, enzymes and adhesion-anchors, accounting for nearly 70% of pharmaceutical drug targets. Difficulties in efficient enrichment, extraction and solubilization still exist because of their relatively low abundance and poor solubility. A simplified membrane protein extraction approach with advantages of user-friendly sample processing procedures, good repeatability and significant effectiveness was developed in the current research for enhancing enrichment and identification of membrane proteins. The approach combining centrifugation and detergent along with LC-MS/MS successfully identified higher proportion of membrane proteins, integral proteins and transmembrane proteins in membrane fraction (76.6%, 48.1%, and 40.6%) than in total cell lysate (41.6%, 16.4%, and 13.5%), and tended to capture membrane proteins with high degree of hydrophobicity and number of transmembrane domains as 486 out of 2106 (23.0%) had GRAVY>0 in membrane fraction, 488 out of 2106 (23.1%) had TMs ≥2. It also provided for improved identification of membrane proteins as more than 60.6% of the commonly-identified membrane proteins in two cell samples were better identified in membrane fraction with higher sequence coverage.
HostingRepository	PRIDE
AnnounceDate	2019-09-27
AnnouncementXML	Submission_2019-09-27_10:43:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Gao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-13 01:19:35	ID requested
⏵ 1	2019-09-27 10:43:47	announced
2	2024-10-22 04:40:27	announced	2024-10-22: Updated project metadata.

Zhou Y, Gao J, Zhu H, Xu J, He H, Gu L, Wang H, Chen J, Ma D, Zhou H, Zheng J, Enhancing Membrane Protein Identification Using a Simplified Centrifugation and Detergent-Based Membrane Extraction Approach. Anal Chem, 90(4):2434-2439(2018) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: Membrane proteins, enrichment, identification, mass spectrometry, sequence coverage

Zhou Hu
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	zhouhu@simm.ac.cn
lab head
Jing Gao
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Science
contact email	jinggao@simm.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD007731

PRIDE project URI

• PRIDE
  1. PXD007731
     1. Label: PRIDE project
     2. Name: Enhancing Membrane Protein Identification Using a Simplified, Centrifugation and Detergent-based Membrane Extraction Approach