PXD007701-1
PXD007701 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Multi-omics and Network Evaluation of Arogenate Dehydratase Knock-out Mutants in Arabidopsis thaliana |
Description | By knocking out genes encoding specific arogenate dehydrase (ADT) enzymes in Arabidopsis, variable reductions in lignin can be achieved. To understand how ADT composition affects plant phenotypes and biomolecular systems, we successfully constructed single and multiple ADT knockout (KO) mutants in Arabidopsis. Using these mutants, a multi-omics (metabolome, transcriptome and proteome) evaluation was conducted using GC- and LC-MS, RNA-Seq, and iTRAQ labeled LC-MS/MS technologies. Identifications include primary and secondary metabolites, transcripts, and proteins in leaf and stem samples taken at 4 weeks of age from 9 KO and wild-type (WT) lines. |
HostingRepository | MassIVE |
AnnounceDate | 2021-03-03 |
AnnouncementXML | Submission_2021-03-03_10:31:14.952.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Matthew Monroe |
SpeciesList | scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; |
ModificationList | Oxidation; Carbamidomethyl; iTRAQ8plex |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-09-08 06:34:55 | ID requested | |
⏵ 1 | 2021-03-03 10:31:15 | announced |
Publication List
no publication |
Keyword List
submitter keyword: multiomics, arogenate dehydratase |
Contact List
Norman G. Lewis | |
---|---|
contact affiliation | Washington State University |
contact email | lewisn@wsu.edu |
lab head | |
Matthew Monroe | |
contact affiliation | Pacific Northwest National Laboratory |
contact email | matthew.monroe@pnnl.gov |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081518/ |