PXD007673 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemical cross-linking mass spectrometry for systems structural biology in mouse heart tissue. |
Description | While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically non-existent. Many protein properties and their involvement in disease pathways are not replicated in cell culture and some complexes are not amenable to purification. Thus, the lack of tissue-level structural information limits molecular-level insight on diseases such as heart failure. Here we demonstrate the application of XL-MS to identify protein structural features and interactions in tissue samples, providing the first systems structural biology insight on protein complexes as they exist in the mouse heart. |
HostingRepository | PRIDE |
AnnounceDate | 2017-11-30 |
AnnouncementXML | Submission_2017-12-11_03:58:10.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Juan Chavez |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | LTQ FT; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-06 05:37:24 | ID requested | |
1 | 2017-11-30 00:56:19 | announced | |
⏵ 2 | 2017-12-11 03:58:11 | announced | Updated publication reference for PubMed record(s): 29199018. |
Publication List
Chavez JD, Lee CF, Caudal A, Keller A, Tian R, Bruce JE, Chemical Crosslinking Mass Spectrometry Analysis of Protein Conformations and Supercomplexes in Heart Tissue. Cell Syst, 6(1):136-141.e5(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Mouse, Heart, Cross-linking, Mass Spectrometry, Structural Biology, Protein-Protein Interactions |
Contact List
James E. Bruce |
contact affiliation | Genome Sciences, University of Washington, USA |
contact email | jimbruce@uw.edu |
lab head | |
Juan Chavez |
contact affiliation | University of Washington |
contact email | jdchavez@uw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007673
- Label: PRIDE project
- Name: Chemical cross-linking mass spectrometry for systems structural biology in mouse heart tissue.