PXD007646-1

PXD007646 is an original dataset announced via ProteomeXchange.

Title	Serial-Omics of Equine Urine
Description	Horse urine is easily collected and contains molecules readily measurable using mass spectrometry that can be used as biomarkers representative of health, disease or drug tampering. This study aimed at analyzing microliter levels of horse urine to purify, identify and quantify proteins, polar metabolites and non-polar lipids. Urine from a healthy 12 year old quarter horse mare on a diet of grass hay and vitamin/mineral supplements with limited pasture access was collected for serial-omics characterization. The urine was treated with methyl tert-butyl ether (MTBE) and methanol to partition into three distinct layers for protein, non-polar lipid and polar metabolite content from a single liquid-liquid extraction and was repeated two times. Each layer was analyzed by high performance liquid chromatography – high resolution tandem mass spectrometry (LC-MS/MS) to obtain protein sequence and relative protein levels as well as identify and quantify small polar metabolites and lipids. The results show 46 urine proteins, many related to normal kidney function, structural and circulatory proteins as well as 474 small polar metabolites but only 10 lipid molecules. Metabolites were mostly related to urea cycle and ammonia recycling as well as amino acid related pathways, plant diet specific molecules, etc. The few lipids represented triglycerides and phospholipids. These data show a complete mass spectrometry based –omics characterization of equine urine from a single 333 uL mid-stream urine aliquot. The data can be used as a baseline for healthy urine composition and analyses can be used to monitor disease progression, health status, monitor drug use, etc.
HostingRepository	PRIDE
AnnounceDate	2018-07-23
AnnouncementXML	Submission_2018-07-23_08:25:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Asara
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	No PTMs are included in the dataset
Instrument	Q TRAP; Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-04 05:59:45	ID requested
⏵ 1	2018-07-23 08:25:28	announced
2	2024-10-22 04:40:12	announced	2024-10-22: Updated project metadata.

Yuan M, Breitkopf SB, Asara JM, Serial-omics characterization of equine urine. PLoS One, 12(10):e0186258(2017) [pubmed]

curator keyword: Biological

submitter keyword: Horse, Urine, Equine, Metabolomics, Proteomics, Lipidomics, Integration, Biomarkers

John M Asara
contact affiliation	Associate Professor of Medicine Harvard Medical School Director, Mass Spectrometry Core Beth Israel Deaconess Medical Center 3 Blackfan Circle CLS 447 office CLS 425 lab Boston, MA 02115 Tel: 617-735-2651
contact email	jasara@bidmc.harvard.edu
lab head
John Asara
contact affiliation	Signal Transduction
contact email	jasara@bidmc.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007646
     1. Label: PRIDE project
     2. Name: Serial-Omics of Equine Urine