PXD007637-2

PXD007637 is an original dataset announced via ProteomeXchange.

Title	Molecular alterations elicited by chronic exposure to cigarette smoke in oral keratinocytes
Description	Smoking is the leading cause of preventable death worldwide. It increases the risk for various diseases including respiratory diseases, vascular diseases and different cancers including lung, oral and bladder cancer. Despite being the leading cause of oral cancer, the molecular mechanisms resulting in malignancy upon cigarette smoke exposure are yet to be fully elucidated. It is crucial to note that disease development is observed upon chronic exposure to cigarette smoke, as opposed to a short-term exposure. Hence, we sought to investigate the effect of chronic smoke exposure on normal oral keratinocytes (OKF6/TERT1). We employed tandem mass tag-based quantitative proteomic and phosphoproteomic approaches to investigate the proteomic and signaling changes in OKF6/TERT1 cells chronically exposed to cigarette smoke compared to untreated cells. LC/MS2 analysis resulted in the quantification of 5,067 proteins among which expression of 360 proteins were found to be dysregulated in at least one replicate. Phosphoproteomic analysis revealed quantification of 3,647 phosphopeptides corresponding to 1,801 proteins. Majority of the dysregulated proteins were seen to be involved in cellular processes such as cell growth, cellular communication and energy metabolism. This study will aid in elucidating the effects of smoking in oral cancer and in the identification of potential candidates molecules which could serve as early detection biomarkers or therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:09.943.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aditi Chatterjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-04 01:38:43	ID requested
1	2018-03-05 01:17:41	announced
⏵ 2	2024-10-22 04:40:18	announced	2024-10-22: Updated project metadata.

10.1007/s12079-017-0442-2;

Rajagopalan P, Nanjappa V, Patel K, Jain AP, Mangalaparthi KK, Patil AH, Nair B, Mathur PP, Keshava Prasad TS, Califano JA, Sidransky D, Gowda H, Chatterjee A, Role of protein kinase N2 (PKN2) in cigarette smoke-mediated oncogenic transformation of oral cells. J Cell Commun Signal, 12(4):709-721(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: chronic exposure,Orbitrap Fusion, carcinogenesis, high-throughput, smoking

Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, 7th floor, Unit-1, Discoverer building, ITPL, Bangalore - 560066, India
contact email	aditixchatterjee@gmail.com
lab head
Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India
contact email	aditixchatterjee@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD007637

PRIDE project URI

• PRIDE
  1. PXD007637
     1. Label: PRIDE project
     2. Name: Molecular alterations elicited by chronic exposure to cigarette smoke in oral keratinocytes