PXD007612 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Maize and Arabidopsis Golgi proteome |
Description | Comparative proteomics of ER-Golgi membranes from etiolated coleoptiles of maize (Zea mays) and young leaves of Arabidopsis (Arabidopsis thaliana) isolated by flotation centrifugation demonstrate differences in the complement of synthases and glycosyl transferases unique to the type of wall made. Additionally, maize Golgi membranes enriched by flotation were separated further by free-flow electrophoresis (FFE), yielding 3009 proteins, of which 229 were known to function in cell wall synthesis or metabolism. A subset of proteins identified after flotation centrifugation were identified in Golgi membranes but failed to enrich in them. Individual classes of proteins associated with cell wall synthesis were asymmetrically distributed across the fractions of Golgi membranes separated by FFE. |
HostingRepository | PRIDE |
AnnounceDate | 2019-04-11 |
AnnouncementXML | Submission_2019-07-08_00:19:57.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD007612 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ikenna Okekeogbu |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Zea mays (Maize); NCBI TaxID: 4577; |
ModificationList | Oxidation; Acetyl; Ethanolyl |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-31 01:25:50 | ID requested | |
1 | 2019-04-11 04:09:18 | announced | |
⏵ 2 | 2019-07-08 00:19:58 | announced | Updated publication reference for PubMed record(s): 30914498. |
Publication List
Okekeogbu IO, Pattathil S, Gonz, á, lez Fern, á, ndez-Ni, ñ, o SM, Aryal UK, Penning BW, Lao J, Heazlewood JL, Hahn MG, McCann MC, Carpita NC, Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures. Plant Cell, 31(5):1094-1112(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Arabidopsis, maize, Golgi, flotation centrifugation, Free-Flow Electrophoresis, FFE, LC-MSMS, Triple TOF |
Contact List
Nicholas Carpita |
contact affiliation | Carpita Lab; Department of Botany & Plant Pathology, Purdue University, USA |
contact email | carpita@purdue.edu |
lab head | |
Ikenna Okekeogbu |
contact affiliation | Purdue University |
contact email | iokekeog@purdue.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007612
- Label: PRIDE project
- Name: Maize and Arabidopsis Golgi proteome