PXD007601 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparison of targeted mass spectrometry techniques with an immunoassay: a case study for HSP90 alpha, part 2 |
Description | We developed 2D-LC-MS/MS-based SRM and PRM assays to measure HSP90 alpha in serum and compared the results to a commercially available immunoassay (ELISA). Serum samples were trypsin-digested and fractionated by SCX chromatography prior to SRM and PRM measurements. PRM data obtained by high-resolution mass spectrometry correlated better with ELISA measurements than SRM data measured on a triple quadrupole mass spectrometer. While all three methods (SRM, PRM, ELISA) were able to quantify HSP90 alpha in serum at the ng/mL level, the use of PRM on a high-resolution mass spectrometer reduced variation. To rule out that the observed differences in SRM and PRM are due to different mass spectrometry systems, the SCX fractions were measured on the same high-resolution instrument (Orbitrap) in the ion trap mode (IT-PRM); such measurements showed that intense co-eluting signals were present in the SRM method, but these interfering peaks were eliminated in the high-resolution PRM mode. Thus, this report shows that it is possible to measure ng/mL levels of HSP90 alpha in a reproducible, selective and sensitive way using PRM. This opens up the possibility to quantify low levels of multiple proteins in complex samples based on a fractionation strategy on tryptic peptides followed by SRM and PRM. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-05 |
AnnouncementXML | Submission_2018-07-05_06:54:06.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD007601 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Coskun Guzel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-30 09:29:55 | ID requested | |
⏵ 1 | 2018-07-05 06:54:07 | announced | |
Publication List
G, ΓΌ, zel C, Govorukhina NI, Stingl C, Dekker LJM, Boichenko A, van der Zee AGJ, Bischoff RPH, Luider TM, . Proteomics Clin Appl, 12(1):(2018) [pubmed] |
Keyword List
curator keyword: Technical, Biomedical |
submitter keyword: Serum, SCX, HSP90, SRM, PRM, ELISA |
Contact List
Theo Luider |
contact affiliation | Head of Laboratory of Neuro-Oncology Neurology Clinical & Cancer Proteomics the Netherlands |
contact email | t.luider@erasmusmc.nl |
lab head | |
Coskun Guzel |
contact affiliation | Erasmus MC |
contact email | c.guzel@erasmusmc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD007601 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007601
- Label: PRIDE project
- Name: Comparison of targeted mass spectrometry techniques with an immunoassay: a case study for HSP90 alpha, part 2