PXD007575-1

PXD007575 is an original dataset announced via ProteomeXchange.

Title	Multiplexed thiol reactivity profiling for target discovery of electrophilic natural products
Description	Electrophilic groups, such as Michael acceptors, expoxides, are common motifs in natural products (NPs). Electrophilic NPs can act through covalent modification of cysteinyl thiols on functional proteins, and exhibit potent cytotoxicity and anti-inflammatory/cancer activities. Here we describe a new chemoproteomic strategy, termed multiplexed thiol reactivity profiling (MTRP), and its use in target discovery of electrophilic NPs. We demonstrate the utility of MTRP by identifying cellular targets of gambogic acid, an electrophilic NP that is currently under evaluation in clinical trials as anticancer agent. Moreover, MTRP enables simultaneous comparison of seven structurally diversified -unsaturated -lactones, which provides insights into the relative proteomic reactivity and target preference of diverse structural scaffolds coupled to a common electrophilic motif and reveals various potential druggable targets with liganded cysteines. We anticipate that this new method for thiol reactivity profiling in a multiplexed manner will find broad application in redox biology and drug discovery.
HostingRepository	PRIDE
AnnounceDate	2017-10-12
AnnouncementXML	Submission_2017-10-12_01:50:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007575
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jing Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; iTRAQ8plex; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-28 07:36:52	ID requested
⏵ 1	2017-10-12 01:50:12	announced
2	2024-10-22 04:02:28	announced	2024-10-22: Updated project metadata.

Tian C, Sun R, Liu K, Fu L, Liu X, Zhou W, Yang Y, Yang J, Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol, 24(11):1416-1427.e5(2017) [pubmed]

curator keyword: Biological

submitter keyword: MTRP;natural products;Chemoproteomics

Jing Yang
contact affiliation	National Center for Protein Science Beijing
contact email	yangjing54@hotmail.com
lab head
Jing Yang
contact affiliation	National Center for Protein Sciences, Beijing
contact email	yangjing54@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007575
     1. Label: PRIDE project
     2. Name: Multiplexed thiol reactivity profiling for target discovery of electrophilic natural products