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PXD007528-1

PXD007528 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal ion suppression limits the potential of mass spectrometry based phosphoproteomics
DescriptionMass spectrometry-based proteomics has become the method of choice to pinpoint and monitor thousands of post-translational modifications, predominately phosphorylation sites, in cellular signaling studies. Critical for achieving this analytical depth is the enrichment for phosphorylated peptides prior to LC-MS analysis. Despite the high prevalence of this modification, the numbers of identified phosphopeptides are lower than those achieved for unmodified peptides, and the cause for this still remains controversial. Here we introduce an effective phosphatase protocol that considerably improves global ionization efficiency and therefore overall sensitivity and coverage of standard phosphoproteomics studies. We demonstrate the power of our method on the model system of Salmonella-infected macrophages by extending the current quantitative picture of immune signaling pathways involved in infection.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:09:06.294.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD007528
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Schmidt
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListPhospho; Oxidation; Carbamidomethyl
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-08-22 03:43:11ID requested
12024-10-07 10:09:07announced
Publication List
Dreier RF, Ahrn, é E, Broz P, Schmidt A, Global Ion Suppression Limits the Potential of Mass Spectrometry Based Phosphoproteomics. J Proteome Res, 18(1):493-507(2019) [pubmed]
10.1021/acs.jproteome.8b00812;
10.6019/PXD007528;
Keyword List
curator keyword: Technical
submitter keyword: macrophages,TMT, phosphoproteomics, infection, ion supression, LC-MS/MS
Contact List
Alexander Schmidt
contact affiliationBiozentrum, University of Basel, Klinglebergstrasse 50/70, CH-4056 Basel
contact emailalex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliationProteomics Core Facility
contact emailalex.schmidt@unibas.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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