PXD007284-1

PXD007284 is an original dataset announced via ProteomeXchange.

Title	Substrate identification of ADAMTS16
Description	Secreted and cell-surface proteases are major mediators of extracellular matrix remodelling, but their mechanisms and regulatory impact are poorly understood. We developed a mass spectrometry approach using cell free ECM produced by mouse Balb/c 3T3 cells in vitro to identify fibronectin as a novel substrate of the secreted metalloprotease ADAMTS16. ADAMTS16 cleaves fibronectin between its (I)5 and (I)6 modules, releasing the N-terminal 30kDa heparin-binding domain essential for fibronectin assembly.
HostingRepository	PRIDE
AnnounceDate	2018-04-23
AnnouncementXML	Submission_2018-04-23_04:10:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rahel Schnellmann
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-16 23:54:57	ID requested
⏵ 1	2018-04-23 04:10:31	announced
2	2024-10-22 04:14:32	announced	2024-10-22: Updated project metadata.

Schnellmann R, Sack R, Hess D, Annis DS, Mosher DF, Apte SS, Chiquet-Ehrismann R, A Selective Extracellular Matrix Proteomics Approach Identifies Fibronectin Proteolysis by A Disintegrin-like and Metalloprotease Domain with Thrombospondin Type 1 Motifs (ADAMTS16) and Its Impact on Spheroid Morphogenesis. Mol Cell Proteomics, 17(7):1410-1425(2018) [pubmed]

curator keyword: Biological

submitter keyword: ADAMTS16, Fibronectin, LC-MS/MS

Suneel S. Apte
contact affiliation	Cleveland Clinic Lerner Research Institute
contact email	aptes@ccf.org
lab head
Rahel Schnellmann
contact affiliation	Friedrich Miescher Institue
contact email	rahel.schnellmann@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD007284

PRIDE project URI

• PRIDE
  1. PXD007284
     1. Label: PRIDE project
     2. Name: Substrate identification of ADAMTS16