PXD007262-3

PXD007262 is an original dataset announced via ProteomeXchange.

Title	Suppressor of IKKepsilon forms direct interactions with cytoskeletal proteins, tubulin and alpha-actinin, linking innate immunity to the cytoskeleton
Description	Suppressor of IKKepsilon (SIKE) is associated with the innate immune system’s type I interferon response through TANK binding kinase 1 (TBK1). Characterized as an endogenous inhibitor of TBK1 when overexpressed in viral infection and pathological cardiac hypertrophic models, mechanistic study revealed SIKE acted as a high affinity substrate of TBK1, but SIKE’s function remains unknown. This study aimed to investigate the SIKE interaction network to identify by association a potential function for SIKE. Our affinity purification/mass spectrometry results showed that SIKE formed interactions with cytoskeletal proteins, nucleic acid-associated proteins, enzymes, and chaperones. In immunofluorescence assays, endogenous SIKE localized to cytosolic puncta in epithelial and myeloid cells whilst in epithelial cells additional staining occurred in stress fiber-like structures and adjacent to the plasma membrane, whereas myeloid cells revealed SIKE associated with nuclear puncta. Using cellular markers, significant co-occurrence of SIKE fluorescence with actin, a-actinin, tubulin, ezrin, FAK and b-catenin was detected. Reciprocal immunoprecipitation and in vitro precipitation assays confirmed a direct SIKE interaction with tubulin and a-actinin. These results indicate that SIKE directly associates with the cytoskeleton thus demonstrating its potential role in mediating cytoskeletal rearrangement necessary in innate immunity but also its ability to link a key catalytic hub, TBK1, to the cytoskeleton to direct TBK1 activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:08.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin Knitter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-14 01:55:43	ID requested
1	2018-05-29 09:18:49	announced
2	2018-07-12 03:57:10	announced	Updated publication reference for PubMed record(s): 29988566.
⏵ 3	2024-10-22 04:14:10	announced	2024-10-22: Updated project metadata.

10.1002/2211-5463.12454;

Sonnenschein HA, Lawrence KF, Wittenberg KA, Slykas FA, Dohleman EL, Knoublauch JB, Fahey SM, Marshall TM, Marion JD, Bell JK, -actinin, linking innate immunity to the cytoskeleton. FEBS Open Bio, 8(7):1064-1082(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: innate immunity, cytoskeleton,SIKE, tubulin, alpha-actinin

Kristina T Nelson
contact affiliation	Chemical and Proteomic Mass Spectrometry Core Facility, Virginia Commonwealth University
contact email	ktnelson@vcu.edu
lab head
Kevin Knitter
contact affiliation	Virginia Commonwealth University
contact email	ksknitter@vcu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD007262

PRIDE project URI

• PRIDE
  1. PXD007262
     1. Label: PRIDE project
     2. Name: Suppressor of IKKepsilon forms direct interactions with cytoskeletal proteins, tubulin and alpha-actinin, linking innate immunity to the cytoskeleton