PXD007243-2

PXD007243 is an original dataset announced via ProteomeXchange.

Title	Comprehensive profiling of lysine ubiquitome reveals diverse functions of lysine ubiquitination in common wheat
Description	Protein ubiquitination, which is a major post-translational modifications that occurs in eukaryotic cells, is involved in diverse biological processes. To date, large-scale profiling of the ubiquitome in common wheat has not been reported, despite its status as the major cereal crop in the world. Here, we performed the first ubiquitome analysis of the common wheat (Triticum aestivum L.) variety, Aikang 58. Overall, 433 lysine modification sites were identified in 285 proteins in wheat seedlings, and four putative ubiquitination motifs were revealed. Moreover, ubiquitylated lysines were found to have a significantly different preference for secondary structures when compared with the all lysines. Bioinformatics analysis revealed that the ubiquitinated proteins were involved in a wide variety of biological processes and diverse subcellular localizations. In accordance with previous studies, proteins related to binding and catalytic activity were predicted to be the preferential targets of lysine ubiquitination. Besides, protein interaction network analysis reveals that diverse interactions are modulated by protein ubiquitination. The results presented herein provide a global view of the ubiquitome in common wheat for the first time and lays a foundation for exploring the physiological role of lysine ubiquitination in wheat and other plants.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:12:47.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ning Zhang
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-10 02:23:05	ID requested
1	2017-11-01 02:27:19	announced
⏵ 2	2024-10-22 04:12:47	announced	2024-10-22: Updated project metadata.

10.1038/s41598-017-13992-y;

Zhang N, Zhang L, Shi C, Tian Q, Lv G, Wang Y, Cui D, Chen F, Comprehensive profiling of lysine ubiquitome reveals diverse functions of lysine ubiquitination in common wheat. Sci Rep, 7(1):13601(2017) [pubmed]

curator keyword: Biological

submitter keyword: Triticum aestivum L.

post-translational modification

lysine ubiquitination

proteomics

mass spectrometry

Feng Chen
contact affiliation	Agronomy College, Henan Agricultural University, China
contact email	chf0088@163.com
lab head
Ning Zhang
contact affiliation	Henan Agricultural University
contact email	zhangning88012@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007243

PRIDE project URI

• PRIDE
  1. PXD007243
     1. Label: PRIDE project
     2. Name: Comprehensive profiling of lysine ubiquitome reveals diverse functions of lysine ubiquitination in common wheat