PXD007215-3

PXD007215 is an original dataset announced via ProteomeXchange.

Title	Analysis of Substrate Specificity of Trypanosoma brucei OSTs by Functional Expression in Yeast
Description	N-linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes. The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to asparagine residues of nascent polypeptide chains is catalyzed by the oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER). Trypanosoma brucei encodes three paralogue single protein OSTs called TbSTT3A, TbSTT3B and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST. We characterized the LLO and polypeptide specificity of all three OST isoforms in the heterologous expression host S. cerevisiae. We demonstrated that TbSTT3A accepted LLO substrates ranging from Man5GlcNAc2 to Man7GlcNAc2. In contrast, TbSTT3B required Man6GlcNAc2 to Glc3Man9GlcNAc2 structures while TbSTT3C did not display any LLO preference. We identified regions within different OST proteins that influence the specificity towards the LLO and polypeptide substrate.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:13:58.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristina Poljak
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-08 05:34:27	ID requested
1	2017-11-03 05:34:29	announced
2	2019-02-12 08:37:38	announced	Updated publication reference for PubMed record(s): 29042445.
⏵ 3	2024-10-22 04:14:03	announced	2024-10-22: Updated project metadata.

10.1074/jbc.M117.811133;

Poljak K, Breitling J, Gauss R, Rugarabamu G, Pellanda M, Aebi M, oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast. J Biol Chem, 292(49):20342-20352(2017) [pubmed]

curator keyword: Biological

submitter keyword: lipid linked oligosaccharide specificity, oligosaccharyltransferase, N-linked glycosylation, glycosylation occupancy analysis,Trypanosoma brucei

Markus Aebi
contact affiliation	ETH Zurich, Institute of Microbiology, Zurich, Switzerland
contact email	markus.aebi@micro.biol.ethz.ch
lab head
Kristina Poljak
contact affiliation	ETH Zurich
contact email	poljakk@ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007215
     1. Label: PRIDE project
     2. Name: Analysis of Substrate Specificity of Trypanosoma brucei OSTs by Functional Expression in Yeast