PXD007169-1

PXD007169 is an original dataset announced via ProteomeXchange.

Title	Modeling acrylamide acute neurotoxicity in zebrafish larvae
Description	Background: Acrylamide (ACR) is a broadly spread neurotoxic chemical of public health concern, as occupational or environmental exposure of humans to ACR may lead to a synaptopathy characterized by ataxia, skeletal muscles weakness and numbness of the extremities. Currently, only the mildly affected patients undergo complete recovery, and identification of new molecules with therapeutic bioactivity against ACR acute neurotoxicity is urgently needed. Objectives: We aimed to develop a zebrafish model for human ACR neurotoxicity. Methods: Adverse effects have been assessed at different levels, including analysis of the motor function (basal locomotor activity, visual motor response, kinematic of the touch-evoked escape response), histopathological evaluation (toluidine blue and whole-mount immunofluorescence), analysis of neural transcriptional markers (qPCR), proteomic analysis (μLC-MS-MS) and neurotransmitters profile (LC-MS/MS). Results: Our results show that zebrafish mimics most of the pathophysiological processes described in humans and mammalian models. Motor function was altered, including the response to sudden changes in light intensity. Specific effects were found on the presynaptic nerve terminals at the neuromuscular junction level, but not on the axonal tracts or myelin sheath integrity. Transcriptional markers of proteins involved in synaptic vesicle cycle were selectively altered, and the proteomic analysis showed that ACR-adducts were formed on cysteine residues of some synaptic proteins. Finally, analysis of neurotransmitters profile showed a significant effect found on cholinergic and dopaminergic systems. Conclusions: Thus, the zebrafish model for ACR acute neurotoxicity is suitable to be used larvae for in vivo high-throughput screening of small molecule libraries for identifying new drugs against this synaptopathy.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-23_16:05:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-02 02:48:01	ID requested
⏵ 1	2018-10-23 16:05:45	announced
2	2024-10-22 04:39:59	announced	2024-10-22: Updated project metadata.

Prats E, G, ó, mez-Canela C, Ben-Lulu S, Ziv T, Padr, ó, s F, Tornero D, Garcia-Reyero N, Tauler R, Admon A, Rald, ú, a D, Modelling acrylamide acute neurotoxicity in zebrafish larvae. Sci Rep, 7(1):13952(2017) [pubmed]

curator keyword: Biological

submitter keyword: neurotoxicity, zebrafish model, acrylamide

Demetrio Raldúa
contact affiliation	2IDAEA-CSIC, Jordi Girona 18, 08034, Barcelona, Spain
contact email	drpqam@cid.csic.es
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@tx.technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007169

PRIDE project URI

• PRIDE
  1. PXD007169
     1. Label: PRIDE project
     2. Name: Modeling acrylamide acute neurotoxicity in zebrafish larvae