PXD007134-1

PXD007134 is an original dataset announced via ProteomeXchange.

Title	Identification and validation of a salivary protein panel to detect heart failure early
Description	Background: Over 26 million people suffer from heart failure (HF) globally. Current diagnosis of HF relies on clinical evaluation, blood assays and imaging techniques at the time of diagnosis. Our aim is to develop a diagnostic assay to detect HF early in at risk individuals within the community using human saliva as a medium, paving the way towards precision medicine. Methods: Saliva samples were collected from healthy controls (n=36) and HF patients (n=75). Salivary proteome profiles were analysed by Sequential Window Acquisition of All Theoretical fragment ion spectra – Mass Spectrometry (SWATH-MS). A total of 738 proteins were quantified and 177 proteins demonstrated significant differences in saliva from HF patients and healthy controls. Candidate biomarkers were chosen based on their abundance and difference between the two cohorts. A multi-protein panel was developed using logistic regression analysis. The diagnostic performance of the multi-protein panel was assessed using the receiver operative characteristic curves. The candidate proteins were further validated technically, using western blot analysis as well as biologically, using an independent cohor.t. Results: A group of six proteins were chosen as potential protein candidates based on their differences in the abundance between the two cohorts. During the validation phase, two of the proteins were eliminated as these did not meet our criteria. The panel consists of four proteins with sensitivity of 83.3%, specificity of 62.5% with an area under ROC curve of 0.78 in discriminating healthy controls from NYHA class I/II HF patients. Conclusion: Analysis of salivary proteome using SWATH-MS analysis revealed novel HF-specific protein candidates when combined into a panel yielding high diagnostic performance. A multi-centre longitudinal clinical trial will be the next step before clinical implementation of this panel.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_11:20:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xi Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-26 02:14:30	ID requested
⏵ 1	2018-10-24 11:20:30	announced
2	2024-10-22 04:39:20	announced	2024-10-22: Updated project metadata.

Zhang X, Walsh T, Atherton JJ, Kostner K, Schulz B, Punyadeera C, Identification and Validation of a Salivary Protein Panel to Detect Heart Failure Early. Theranostics, 7(18):4350-4358(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Heart failure

Saliva

diagnosis

mass spectrometry

multi-biomarker strategies

Chamindie Punyadeera
contact affiliation	The School of Biomedical Sciences, Queesnland University of Technology, Australia
contact email	chamindie.punyadeera@qut.edu.au
lab head
Xi Zhang
contact affiliation	Institute of Health and Biomedical Innovation, Queensland University of Technology
contact email	x78.zhang@hdr.qut.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007134
     1. Label: PRIDE project
     2. Name: Identification and validation of a salivary protein panel to detect heart failure early