PXD007061-2

PXD007061 is an original dataset announced via ProteomeXchange.

Title	Haloferax volcanii proteome turn-over analysis of a LonB mutant and co-IP results with anti-LonB
Description	The proteome turnover was examined in a conditional LonB mutant (HVLON3) of the model euryarchaeon H. volcanii under reduced (- trp) and nearly physiological (+ trp) LonB levels. HVLON3 was initially grown in minimal medium containing 14NH4Cl as nitrogen source in absence of trp and then switched to 15N-medium with/without trp (± Lon) to monitor 15N-label incorporation into newly synthesized proteins over time. In parallel, degradation of 14N-labeled proteins was estimated by comparing different time points of this culture via an internal standard grown on 13C-glucose. Protein samples (membrane and cytoplasm fractions) were processed by SDS-PAGE, digested with trypsin and analyzed by LC-MS/MS. Proteins were identified with Sequest and protein turnover, as well as statistical analysis, was achieved with the online platform QuPE. In addition, an in vivo crosslinking assay coupled to immunoprecipitation with anti-Lon antibody was performed in the H. volcanii H26 wt strain to detect interactions between LonB and its endogenous targets.
HostingRepository	jPOST
AnnounceDate	2018-02-19
AnnouncementXML	Submission_2018-02-19_05:56:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Micaela Cerletti
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid; 2-pyrrolidone-5-carboxylic acid (Gln); unknown modification: no fixed modification; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-17 05:22:01	ID requested
1	2018-02-19 05:54:51	announced
⏵ 2	2018-02-19 05:56:07	announced	Updated PubMed.
3	2018-09-19 00:17:09	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 17:28:15	announced	2021-02-04: Updated FTP location.
5	2022-09-18 04:00:25	announced	2022-09-18: Updated FTP location.

Cerletti M, Paggi R, Troetschel C, Ferrari MC, Guevara CR, Albaum S, Poetsch A, De Castro R, LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. J Proteome Res, 17(3):1158-1171(2018) [pubmed]

submitter keyword: LonB protease, Haloferax volcanii, proteome turnover, IP, substrates

Rosana De Castro
lab head
Micaela Cerletti
contact affiliation	IIB-UNMdP
dataset submitter

jPOST dataset URI