PXD007038 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SEC-SWATH-MS HEK293 |
Description | Increasingly, biochemical co-fractionation-based approaches are used to study interactomes and protein complexes at high throughput. The devised methods facilitate the qualitative assignment and prediction of hundreds of putative cellular assemblies in one experiment and without dependency on genetic engineering to introduce affinity tags. The present dataset consists of a native proteome extracted by mild lysis from the HEK293 cell line and fractionated into 80 fractions along high resolution size exclusion chromatography, and each fraction analyzed via bottom-up SWATH mass spectrometry. In multi-tiered targeted analysis, from this fragment ion level chromatographic data, quantitative complex assembly information of the proteome is reconstructed in three steps, (i) peptide-centric detection of peptide analytes within each SEC fraction based on fragment ion co-elution groups; (ii) protein-centric detection of protein elution in SEC based on fragment peptide co-elution groups in SEC; (iii) complex-centric detection and quantification of protein complexes and -variants based on component subunit protein co-elution groups in SEC. The data delineate a global picture of quantitative complex formation within a human proteome, including deconvolution of novel subversions and assembly intermediates of critical cellular complexes with essential functions. |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-26 |
AnnouncementXML | Submission_2018-06-26_02:37:52.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Moritz Heusel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-07-13 13:18:56 | ID requested | |
⏵ 1 | 2018-06-26 02:37:53 | announced | |
2 | 2018-06-28 01:05:24 | announced | Updated publication reference for DOI(s): 10.1101/323683. |
3 | 2019-01-11 06:26:32 | announced | Updated project metadata. |
4 | 2019-08-19 07:06:58 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: Size exclusion chromatography, SWATH, Mass Spectrometry, protein complexes |
Contact List
Rudolf Aebersold |
contact affiliation | Institute of Molecular Systems Biology D-BIOL ETH Zurich Switzerland |
contact email | aebersold@imsb.biol.ethz.ch |
lab head | |
Moritz Heusel |
contact affiliation | ETHZ |
contact email | heusel@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD007038 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007038
- Label: PRIDE project
- Name: SEC-SWATH-MS HEK293