PXD007036-2

PXD007036 is an original dataset announced via ProteomeXchange.

Title	Trafficking of Ectopic ATP Synthase via Mitochondrial Fission
Description	Ectopic ATP synthase is a functional onco-protein increases cell proliferation when transported to plasma membrane of cancer cells. Our previous study performed large scale gene silencing screening indicated ER and mitochondrial transport pathways may lead to ectopic ATP synthase expression. Silencing dynamin-related protein 1 (Drp1), mitofusin-1 (Mfn1) and Parkin affected ectopic ATP synthases expression. However, the underlying trafficking mechanism is poorly understood. Here, we analyzed our membrane and mitochondrial proteome of lung cancer A549 cells and found that both nuclear-encoded ATP synthase subunits and mitochondrial-encoded components-ATP6 translocated to cell surface, indicating that ATP synthase subunits assembled in mitochondria. Furthermore, serum starvation enhanced ATP synthase translocation to plasma membrane, Mdivi-1, a chemical inhibitor of the mitochondrial fission protein Drp1, rescued the phenomena. Additionally, image quantification of mitochondria, showing that mitochondrial fission preference cells expressed more eATP synthase. Therefore, we proposed that eATP synthase trafficking may be related to mitochondrial dynamics. Additionally, ICC and flow cytometry revealed the expression of a critical transcription factor associated with high-risk neuroblastoma, MYCN, correlated with eATP synthase expression. To better understand whether MYCN mediated mitochondrial fission and affected ATP synthase trafficking, we first analyzed MYCN ChIP-sequencing data and found Drp1, Mfns and Parkin possessed the consensus DNA-binding motif of MYCN. Further high-resolution image analysis showed higher mitochondrial fission and eATP synthase expression in MYCN-amplified neuroblastoma. Last, silencing MYCN reduced the fission level by detecting DRP1. In summary, we suggest that trafficking of ectopic ATP synthase may via mitochondrial dynamics.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:34.015.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min-Chun Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-13 01:19:11	ID requested
1	2023-05-10 08:42:23	announced
⏵ 2	2023-11-14 08:45:44	announced	2023-11-14: Updated project metadata.

Chang YW, Tony Yang T, Chen MC, Liaw YG, Yin CF, Lin-Yan XQ, Huang TY, Hou JT, Hung YH, Hsu CL, Huang HC, Juan HF, Spatial and temporal dynamics of ATP synthase from mitochondria toward the cell surface. Commun Biol, 6(1):427(2023) [pubmed]

submitter keyword: Ectopic ATP synthases

proteome

protein trafficking

mitochondrial fission

image analysis

Hsueh-Fen Juan
contact affiliation	Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
contact email	yukijuan@gmail.com
lab head
Min-Chun Chen
contact affiliation	National Taiwan University
contact email	r04b43014@g.ntu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007036
     1. Label: PRIDE project
     2. Name: Trafficking of Ectopic ATP Synthase via Mitochondrial Fission