PXD006934 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative analyses of data independent acquisition mass spectrometric approaches: DIA, WiSIM-DIA and directDIA |
Description | With a multitude of DIA approaches developed recently, many of their features are not yet well explored. In this study we make use of a biologically relevant sample, the synaptosome, as a model to compare the spectral libraries generated by (1) DDA solely on Orbitrap (OT), (2) Orbitrap for MS1 scan and ion trap for MS/MS acquisition (IT), and (3) directDIA derived from DIA data. Next, we compared the number of spectral library features recovered, and quality of quantification, by DIA and WiSIM-DIA, and suggest future improvement. |
HostingRepository | PRIDE |
AnnounceDate | 2017-11-20 |
AnnouncementXML | Submission_2017-11-20_06:09:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Frank Koopmans |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | methylthiolated residue; monohydroxylated residue; acetylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-07-11 04:35:59 | ID requested | |
⏵ 1 | 2017-11-20 06:09:45 | announced | |
Publication List
Koopmans F, Ho JTC, Smit AB, Li KW, Comparative Analyses of Data Independent Acquisition Mass Spectrometric Approaches: DIA, WiSIM-DIA, and Untargeted DIA. Proteomics, 18(1):(2018) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: LC-MS/MS, DDA, DIA, WiSIM-DIA, WiSIM, directDIA |
Contact List
Ka Wan Li |
contact affiliation | Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, VU Amsterdam, Amsterdam, The Netherlands |
contact email | k.w.li@vu.nl |
lab head | |
Frank Koopmans |
contact affiliation | VU University |
contact email | frank.koopmans@vu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006934 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006934
- Label: PRIDE project
- Name: Comparative analyses of data independent acquisition mass spectrometric approaches: DIA, WiSIM-DIA and directDIA