PXD006877-1

PXD006877 is an original dataset announced via ProteomeXchange.

Title	Haloferax volcanii SILAC study of Oxidative stress
Description	SILAC was used to monitor proteome changes to Haloferax volcanii after treatment with oxidizing agent NaOCl. SILAC amino acids used were Lysine(+8) and Arginine(+6). For treatment and control groups 4 replicates were used. In replicates 1 and 3, the control group had supplementation of both light lysine and argninie to the medium and the treatment group was supplemented with both heavy lysine(+8) and arginine(+6). In replicates 2 and 4, the labeling was switched, with the control containing heavy lysine(+8) and arginine(+6) and the treatement was supplemented with light lysine and arginine. After treatment, cells were mixed at a 1:1 ratio (control:treatment) and proteins were extracted and digested with trypsin. For the incorporation test, cells were grown in meduim containing heavy lysine(+8) and argnine(+6) and allowed to grow for 24 hours, then subcultured twice, sequentially after 24 h of growth. To test for incorporation of the heavy amino acids, cells were harvested, proteins extracted, and digested with trypsin.
HostingRepository	PRIDE
AnnounceDate	2018-01-09
AnnouncementXML	Submission_2018-01-09_03:52:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lana McMillan
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	methylthiolated residue; 6x(13)C: 2x(15)N labeled L-lysine; 5x(13)C labeled L-proline; monohydroxylated residue; acetylated residue; 6x(13)C labeled L-arginine
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-06 01:17:16	ID requested
⏵ 1	2018-01-09 03:52:52	announced
2	2024-10-22 04:39:42	announced	2024-10-22: Updated project metadata.

McMillan LJ, Hwang S, Farah RE, Koh J, Chen S, Maupin-Furlow JA, Multiplex quantitative SILAC for analysis of archaeal proteomes: a case study of oxidative stress responses. Environ Microbiol, 20(1):385-401(2018) [pubmed]

curator keyword: Biological

submitter keyword: Archaea, SILAC, quantitative proteomics, Haloferax volcanii, Oxidative stress, Sodium hypochlorite

Julie A. Maupin-Furlow
contact affiliation	Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, Genetics Institute, University of Florida, Gainesville, Florida, 32611
contact email	jmaupin@ufl.edu
lab head
Lana McMillan
contact affiliation	University of Florida
contact email	lmcmillan@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006877

PRIDE project URI

• PRIDE
  1. PXD006877
     1. Label: PRIDE project
     2. Name: Haloferax volcanii SILAC study of Oxidative stress