PXD006876-1

PXD006876 is an original dataset announced via ProteomeXchange.

Title	Foetal and adult HSPCs
Description	Haematopoietic stem and progenitor cells (HSPCs) in the foetus and adult possess distinct molecular landscapes that regulate cell fate and change their susceptibility to initiation and progression of haematopoietic malignancies. The proteomic programs that govern these differences remain elusive. In this study, we have utilized a mass spectrometry-based quantitative proteomics approach to comprehensively describe and compare the proteome of foetal and adult HSPCs. We found that the proteome of foetal HSPCs is relatively simple, characterized by proteins involved in cell cycle and cell proliferation, while their adult counterparts are defined by a larger set of proteins that are involved in more diverse cellular processes. These adult characteristics include an arsenal of proteins important for viral and bacterial defence, as well as protection against ROS-induced protein oxidation. Our further analyses of Type I interferon signalling shows that foetal HSPCs are sensitive to Interferon a (IFNa), which impairs their production of mature lymphoid cells, whereas stimulation with IFNa to the pregnant mother enhances the production of early progenitors from foetal HSCs. Our results provide new and important insights into the molecular landscape of foetal and adult haematopoiesis that advance our understanding of normal and malignant haematopoiesis during foetal and adult life.
HostingRepository	PRIDE
AnnounceDate	2017-12-12
AnnouncementXML	Submission_2017-12-11_23:59:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Hansson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	4x(2)H labeled dimethylated residue; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-06 01:16:57	ID requested
⏵ 1	2017-12-11 23:59:31	announced
2	2017-12-18 01:16:10	announced	Updated publication reference for PubMed record(s): 29241553.
3	2024-10-22 04:39:44	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Mouse, haematopoiesis, foetal liver, adult bone marrow

Jenny Hansson
contact affiliation	Lund Stem Cell Center, Division of Molecular Hematology, Lund University, Lund, Sweden
contact email	jenny.hansson@med.lu.se
lab head
Jenny Hansson
contact affiliation	Lund University
contact email	jehansson@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD006876

PRIDE project URI

• PRIDE
  1. PXD006876
     1. Label: PRIDE project
     2. Name: Foetal and adult HSPCs