PXD006869-1

PXD006869 is an original dataset announced via ProteomeXchange.

Title	SWATH-MS analysis of transgenic tobacco overexpressing 3-hydroxy-3-methylglutaryl-CoA synthase
Description	3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is the second enzyme in the mevalonate pathway. While the recombinant Brassica juncea HMGS1 (BjHMGS1) mutant S359A displayed 10-fold higher enzyme activity than wild-type (wt) BjHMGS1, transgenic tobacco overexpressing S359A (OE-S359A) exhibited greater sterol content, growth rate and seed yield than OE-wtBjHMGS1. To explore the mechanism of HMGS and its mutant (S359A) in promotion of plant growth, SWATH-MS quantitative proteomics analysis was performed.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_06:39:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pan Liao
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-05 07:39:20	ID requested
⏵ 1	2021-01-04 06:39:14	announced
2	2024-10-22 05:17:21	announced	2024-10-22: Updated project metadata.

Liao P, Lung SC, Chan WL, Hu M, Kong GK, Bach TJ, Hao Q, Lo C, Chye ML, Overexpression and Inhibition of 3-Hydroxy-3-Methylglutaryl-CoA Synthase Affect Central Metabolic Pathways in Tobacco. Plant Cell Physiol, 62(1):205-218(2021) [pubmed]

curator keyword: Biological

submitter keyword: Nicotiana tabacum, HMGS, SWATH, proteomics analysis

Mee-Len Chye
contact affiliation	Plant molecular biology laboratory, School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China
contact email	mlchye@hku.hk
lab head
Pan Liao
contact affiliation	The University of Hong Kong
contact email	h1092030@connect.hku.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD006869

PRIDE project URI

• PRIDE
  1. PXD006869
     1. Label: PRIDE project
     2. Name: SWATH-MS analysis of transgenic tobacco overexpressing 3-hydroxy-3-methylglutaryl-CoA synthase