PXD006857-1

PXD006857 is an original dataset announced via ProteomeXchange.

Title	MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter
Description	Most E2F-binding sites repress transcription through the recruitment of Retinoblasoma (RB) family members until the end of the G1 cell-cycle phase. Although the MYB promoter contains an E2F-binding site, its transcription is activated shortly after the exit from quiescence, before RB family members inactivation, by unknown mechanisms. We had previously uncovered a nuclear factor distinct from E2F, Myb-sp, whose DNA-binding site overlapped the E2F element and had hypothesized that this factor might overcome the transcriptional repression of MYB by E2F-RB family members. We have purified Myb-sp and discovered that Myc-associated zinc finger proteins (MAZ) are major components. We show that various MAZ isoforms are present in Myb-sp and activate transcription via the MYB-E2F element. Moreover, while forced RB or p130 expression repressed the activity of a luciferase reporter driven by the MYB-E2F element, co-expression of MAZ proteins not only reverted repression, but also activated transcription. Finally, we show that MAZ binds the MYB promoter in vivo, that its binding site is critical for MYB transactivation, and that MAZ knockdown inhibits MYB expression during the exit from quiescence. Together, these data indicate that MAZ is essential to bypass MYB promoter repression by RB family members and to induce MYB expression.
HostingRepository	PRIDE
AnnounceDate	2017-10-05
AnnouncementXML	Submission_2017-10-05_00:13:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006857
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-03 06:53:40	ID requested
⏵ 1	2017-10-05 00:13:23	announced

Á, lvaro-Blanco J, Urso K, Chiodo Y, Mart, í, n-Cort, á, zar C, Kourani O, Arco PG, Rodr, í, guez-Mart, í, nez M, Calonge E, Alcam, í J, Redondo JM, Iglesias T, Campanero MR, MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter. Nucleic Acids Res, 45(17):9960-9975(2017) [pubmed]

curator keyword: Biological

submitter keyword: maz, myb, quiescence, Retinoblasoma

Miguel R. Campanero
contact affiliation	Tenured Scientist and Lab Director Instituto de Investigaciones Biomedicas CSIC-UAM
contact email	mcampanero@iib.uam.es
lab head
Sergio Ciordia
contact affiliation	National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006857
     1. Label: PRIDE project
     2. Name: MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter