PXD006832-1

PXD006832 is an original dataset announced via ProteomeXchange.

Title	PROST: A set of 40 peptide standards for retention time indexing, column performance monitoring and collision energy calibration
Description	We describe PROST (ProteomeTools Standard), a set of 40 synthetic peptides that span the entire hydrophobicity range of tryptic digests, enabling not only accurate determination of retention time indices but also monitoring of chromatographic separation performance over time. The fragmentation characteristics of the peptides can also be used to calibrate and compare collision energies between mass spectrometers. The sequences of all selected peptides do not occur in any natural protein, thus eliminating the need for stable isotope labeling and allowing straightforward integration into standard peptide identification strategies employing database searching or spectral library matching.
HostingRepository	PRIDE
AnnounceDate	2017-09-07
AnnouncementXML	Submission_2017-09-07_08:08:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Zolg
SpeciesList	scientific name: artificial sequences; NCBI TaxID: 81077;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-29 07:40:12	ID requested
⏵ 1	2017-09-07 08:08:44	announced
2	2017-11-16 07:06:00	announced	Updated project metadata.
3	2024-10-22 04:38:38	announced	2024-10-22: Updated project metadata.

Zolg DP, Wilhelm M, Yu P, Knaute T, Zerweck J, Wenschuh H, Reimer U, Schnatbaum K, Kuster B, PROCAL: A Set of 40 Peptide Standards for Retention Time Indexing, Column Performance Monitoring, and Collision Energy Calibration. Proteomics, 17(21):(2017) [pubmed]

curator keyword: Technical

submitter keyword: synthetic peptide, retention time calibration, ProteomeTools, retention time kit, peptide standard

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich
contact email	kuster@tum.de
lab head
Daniel Zolg
contact affiliation	Technical University of Munich
contact email	daniel.zolg@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006832

PRIDE project URI

• PRIDE
  1. PXD006832
     1. Label: PRIDE project
     2. Name: PROST: A set of 40 peptide standards for retention time indexing, column performance monitoring and collision energy calibration