PXD006794-1
PXD006794 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment |
| Description | The oviductal microenvironment is a site for key events that involve gamete maturation, fertilization and early embryo development. Secretions into the oviductal lumen by either the lining epithelium or by transudation of plasma constituents are known to contain elements conducive for reproductive success. Although previous studies have identified some of these factors involved in reproduction, knowledge of secreted proteins in the oviductal fluid remains rudimentary with limited definition of function even in extensively studied species like cattle. In this study, we used a shotgun proteomics approach followed by bioinformatics sequence prediction to identify secreted proteins present in the bovine oviductal fluid (in vivo) and secretions from the bovine oviductal epithelial cells (in vitro). From a total of 2087 proteins identified, 266 proteins could be classified as secreted, 109 (41%) of which were common for both in vivo and in vitro conditions. Pathway analysis indicated different classes of proteins that included growth factors, metabolic regulators, immune modulators, enzymes, and extracellular matrix components. Functional analysis revealed mechanisms in the oviductal lumen linked to immune homeostasis, gamete maturation, fertilization and early embryo development. These results point to several novel components that work together with known elements mediating functional homeostasis, and highlight the diversity of machinery associated with oviductal physiology and early events in cattle fertility. |
| HostingRepository | MassIVE |
| AnnounceDate | 2017-11-06 |
| AnnouncementXML | Submission_2017-11-06_10:04:49.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Bos taurus; common name: cattle; NCBI TaxID: 9913; |
| ModificationList | Oxidation; iodoacetamide derivatized residue; Deamidated |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-06-23 11:26:00 | ID requested | |
| ⏵ 1 | 2017-11-06 10:04:49 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Oviducts |
Contact List
| Vimal Selvaraj | |
|---|---|
| contact affiliation | Cornell |
| contact email | vs88@cornell.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081192 |
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