PXD006687-2

PXD006687 is an original dataset announced via ProteomeXchange.

Title	A Proteomic Approach to Understanding the Pathogenesis of Idiopathic Macular Hole Formation
Description	Idiopathic macular holes (IMH) are full-thickness defects of retinal tissue that cause severe vision loss due to disruption of the anatomic fovea. Abnormal vitreous traction is involved in the formation of macular holes. Both glial cells and hyalocytes contribute to epiretinal membrane formation in IMH. In order to gain further insight into the pathophysiology of IMH, we conducted a discovery phase investigation of the vitreous proteome in four patients with macular holes and six controls using one-dimensional gel fractionation and liquid chromatography-tandem mass spectrometry analyses on an Orbitrap Elite mass spectrometer. Of a total of 5912 vitreous proteins, 32 proteins had increased and 39 proteins had decreased expression in IMH compared with controls, using a false discovery rate approach with p-value <0.001 and q value <0.05. IMH was associated with increased expression of proteins in the complement pathway, α-2-macroglobulin, a major inducer of Müller glial cell migration, fibrinogen, and extracellular matrix proteins, and decreased expression of proteins involved in protein folding and actin filament binding. A proteomic approach revealed proteins and biological pathways that may be involved in the pathogenesis of IMH and could be targeted for future studies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:35.497.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006687
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pingbo Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-09 03:51:37	ID requested
1	2018-04-11 07:14:42	announced
⏵ 2	2024-10-22 04:38:37	announced	2024-10-22: Updated project metadata.

10.1186/s12014-017-9172-y;

Zhang P, Zhu M, Zhao Y, Qian J, Dufresne C, Turner R, Semba RD, Solomon SD, A proteomic approach to understanding the pathogenesis of idiopathic macular hole formation. Clin Proteomics, 14():37(2017) [pubmed]

10.6019/PXD006687;

ProteomeXchange project tag: EyeOME (B/D-HPP), Human Proteome Project

curator keyword: Biomedical

submitter keyword: eye, proteomics, vitreous, idiopathic macular hole, retina

Richard D. Semba
contact affiliation	Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD
contact email	rdsemba@jhmi.edu
lab head
Pingbo Zhang
contact affiliation	Johns Hopkins University
contact email	pzhang7@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006687
     1. Label: PRIDE project
     2. Name: A Proteomic Approach to Understanding the Pathogenesis of Idiopathic Macular Hole Formation