PXD006680-1

PXD006680 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells
Description	4-Oxo-2-nonenal (ONE) derived from lipid peroxidation modifies nucleophiles and transduces redox signaling by its reactions with proteins. However, the molecular interactions between ONE and complex proteomes and their dynamics in situ remain largely unknown. Here we de-scribe a quantitative chemoproteomic analysis of protein adduction by ONE in cells, in which the cellular target profile of ONE is mimicked by its alkynyl surrogate. The analysis reveals four types of ONE-derived modifications in cells, including ketoamide and Schiff-base adducts to lysine, Michael adducts to cysteine, and a novel pyrrole adducts to cysteine. ONE-derived adducts co-localize and crosstalk with many histone marks and redox sensitive sites. All four types of modifications derived from ONE can be reversed site-specifically in cells. In summary, our study provides much-needed mechanistic insights into the cellular signaling and potential toxicities associated with this important lipid derived electrophile.
HostingRepository	PRIDE
AnnounceDate	2022-02-24
AnnouncementXML	Submission_2022-02-24_05:35:21.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-08 03:37:40	ID requested
⏵ 1	2022-02-24 05:35:21	announced
2	2023-11-14 08:49:24	announced	2023-11-14: Updated project metadata.

10.1074/MCP.RA117.000116;

curator keyword: Biological

submitter keyword: Chemoproteomics, 4-Oxo-2-nonenal,Click chemistry

Jing Yang
contact affiliation	NCPSB, China
contact email	yangjing54@hotmail.com
lab head
Jing Yang
contact affiliation	National Center for Protein Sciences, Beijing
contact email	yangjing54@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006680
     1. Label: PRIDE project
     2. Name: Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells