PXD006675-3

PXD006675 is an original dataset announced via ProteomeXchange.

Title	Region and cell-type resolved quantitative proteomic map of the human heart and its application to atrial fibrillation
Description	The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantified over 11,000 proteins in this high dynamic range tissue. We combined copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identified 25 cellular receptors as potential new cell surface markers. Application of our heart map to an atrial fibrillation study revealed signatures of distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:05.885.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-08 01:30:38	ID requested
1	2017-11-14 06:15:27	announced
2	2019-02-12 08:28:11	announced	Updated publication reference for PubMed record(s): 29133944.
⏵ 3	2024-10-22 04:39:08	announced	2024-10-22: Updated project metadata.

Doll S, Dre, ß, en M, Geyer PE, Itzhak DN, Braun C, Doppler SA, Meier F, Deutsch MA, Lahm H, Lange R, Krane M, Mann M, Region and cell-type resolved quantitative proteomic map of the human heart. Nat Commun, 8(1):1469(2017) [pubmed]

10.1038/s41467-017-01747-2;

curator keyword: Biological, Biomedical

submitter keyword: Heart, proteomics, mass spectrometry

Matthias Mann
contact affiliation	Department of Proteomics and Signal Transduction Max-Planck Institute of Biochemistry Germany
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006675

PRIDE project URI

• PRIDE
  1. PXD006675
     1. Label: PRIDE project
     2. Name: Region and cell-type resolved quantitative proteomic map of the human heart and its application to atrial fibrillation