PXD006671-2

PXD006671 is an original dataset announced via ProteomeXchange.

Title	Proteomic landscape of murine intervertebral disc reveals mechanisms involved in homeostasis and disc structure maintenance
Description	To fully apprehend the complex mechanisms responsible for intervertebral disc (IVD) degeneration, one needs to gain a deeper understanding of what characterizes a healthy disc. Using a quantitative proteomic approach, we analyzed methodically the differences existing between IVD genders, levels and components. A total of 2776 proteins were identified and we showed that cross regional but not gender variation existed along the mouse spine. Components comparison established CD109, CD81 and Col12a1 as novel NP and AF markers. NP cell clustering involved Cdh2 and tight junctions whereas early phase of adaptation to hypertonicity, the regulation of cell volume, was ensured by Slc12a2 and Wnk1. AF cells were protected from oxidative stress by expressing Atox1 and Sod3. Finally notochordal-like cells vacuoles were not acidic nor lipid droplets. Altogether, our study provides a first comprehensive landscape of the biology of a healthy murine IVD and identifies mechanisms involved in homeostasis and structure maintenance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:25:17.716.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MATEUSZ KUDELKO
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-07 23:06:26	ID requested
1	2021-09-07 04:09:09	announced
⏵ 2	2024-10-22 05:25:19	announced	2024-10-22: Updated project metadata.

10.1016/j.mbplus.2021.100082;

Kudelko M, Chen P, Tam V, Zhang Y, Kong OY, Sharma R, Au TYK, To MK, Cheah KSE, Chan WCW, Chan D, PRIMUS: Comprehensive proteomics of mouse intervertebral discs that inform novel biology and relevance to human disease modelling. Matrix Biol Plus, 12():100082(2021) [pubmed]

curator keyword: Biological

submitter keyword: Mouse intervertebral disc, nucleus pulposus, annulus fibrosus, proteomic, homeostasis, structure maintenance

Prof Danny Chan
contact affiliation	Endowed Professor, School of Biomedical Sciences, THe University of Hong Kong
contact email	chand@hku.hk
lab head
MATEUSZ KUDELKO
contact affiliation	HKU Biochemistry
contact email	mkudelko@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD006671

PRIDE project URI

• PRIDE
  1. PXD006671
     1. Label: PRIDE project
     2. Name: Proteomic landscape of murine intervertebral disc reveals mechanisms involved in homeostasis and disc structure maintenance