PXD006642-1

PXD006642 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the liver isolated from the double (apolipoprotein E and endothelial nitric oxide synthase) knockout mice reveals decreased expression of major urinary proteins (MUPs)
Description	Nitric oxide (NO) is a crucial gaseous signaling molecule engaged in a variety of physiological and pathological processes. It is produced by three isoenzymes called nitric oxide synthases (NOS): neuronal, inducible and endothelial NOS (eNOS). eNOS-derived NO plays an important role in endothelium-dependent vasodilation as well as in lipid and glucose homeostasis in the liver. In addition, it has been shown that NO exert a beneficial effect on mitochondrial biogenesis and respiration. Thus, the aim of our study was to used iTRAQ-based quantitative proteomics to investigate the changes in protein expression in the mitochondrial and cytosolic fractions isolated from the liver of the double (apolipoprotein E (apoE) and eNOS) knockout (apoE/eNOS-DKO) mice as compared to apoE-/- mice – an animal model of atherosclerosis and hepatic steatosis. Collectively, our proteomic approach revealed the increased expression of proteins related to gluconeogenesis, fatty acids and cholesterol biosynthesis as well as the decreased expression of proteins participated in triglyceride breakdown, cholesterol transport, protein transcription & translation and processing in endoplasmic reticulum (ER). Moreover, one of the most down-regulated proteins were major urinary proteins (MUPs), which are abundantly expressed in the liver and are involved in the regulation of lipid and glucose metabolism as well as mitochondrial function. However, the exact functional consequences of the revealed alterations require further investigation.
HostingRepository	PRIDE
AnnounceDate	2018-03-05
AnnouncementXML	Submission_2018-03-05_01:20:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aneta Stachowicz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-01 01:38:30	ID requested
⏵ 1	2018-03-05 01:20:08	announced

Stachowicz A, Olszanecki R, Suski M, Wi, ś, niewska A, Ku, ś K, Bia, ł, as M, Jawie, ń J, Korbut R, Quantitative proteomics reveals decreased expression of major urinary proteins in the liver of apoE/eNOS-DKO mice. Clin Exp Pharmacol Physiol, 45(7):711-719(2018) [pubmed]

curator keyword: Biological

submitter keyword: MUP, apolipoprotein E, eNOS, liver, proteomics

Rafał Olszanecki
contact affiliation	Jagiellonian University Medical College
contact email	mfolszan@cyf-kr.edu.pl
lab head
Aneta Stachowicz
contact affiliation	Jagiellonian University Medical College
contact email	stachowicz.aneta@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006642
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the liver isolated from the double (apolipoprotein E and endothelial nitric oxide synthase) knockout mice reveals decreased expression of major urinary proteins (MUPs)