PXD006612-1

PXD006612 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analyses of Cysteine Redox in High-fat-fed and Fasted Mouse Livers: Implications for Liver Metabolic Homeostasis
Description	Intensive oxidative stress occurs during high-fat-diet-induced hepatic fat deposition, suggesting a critical role for redox signaling in liver metabolism. Intriguingly, lines of evidence show that fasting could also result in redox profile changes, largely through reduced oxidant and/or increased anti-oxidant levels. However, a comprehensive landscape of redox-modified hepatic substrates is lacking, thus hindering our understanding of liver metabolic homeostasis. In this study, we employed a proteomic approach which combines iodoTMT and nanoLC-MS/MS to quantitatively probe the effects of high-fat-feeding and fasting on in vivo redox-based cysteine modifications. Collectively, we identified 1258 redox cysteine sites in 603 proteins and quantified 846 sites in 403 proteins.
HostingRepository	PRIDE
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-03-03_01:17:23.664.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhou lianqi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-29 04:41:08	ID requested
⏵ 1	2022-03-03 01:17:24	announced
2	2023-11-14 08:49:09	announced	2023-11-14: Updated project metadata.

10.1021/acs.jproteome.7b00431;

curator keyword: Biological

submitter keyword: High-fat-fed, Proteomic

Lei Zhou
contact affiliation	State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, P.R. China
contact email	zhoulei@gxu.edu.cn
lab head
zhou lianqi
contact affiliation	ptm-biolab
contact email	lianqi_zhou@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006612
     1. Label: PRIDE project
     2. Name: Proteomic Analyses of Cysteine Redox in High-fat-fed and Fasted Mouse Livers: Implications for Liver Metabolic Homeostasis