PXD006567-1

PXD006567 is an original dataset announced via ProteomeXchange.

Title	An Insight into Glyco-microheterogeneity of Plasma von Willebrand Factor (vWF) by Mass Spectrometry
Description	Von Willebrand Factor (vWF), a plasma glycoprotein, plays essential roles in primary hemostasis, in cooperation with other coagulation factors. The glycosylation affects many biological phases during the protein life-cycle. In this work, we comprehensively characterized the microheterogeneity at all probable N-glycosites in simultaneous with O-glycosites, beside the N-occupancy estimation.An RP-LC-MS/MS system functionalized with CID and HCD tandem mass techniques was utilized. Our results showed gradual glycosylation occupancy along the protein backbone. A total of 181 glycoforms, 173 N-froms and 8 O-forms, were detected and specified into plasma vWF glycosites. Given subtle characterization of site-specific glycoforms is critical for profound understanding the protein biological functions and activity.
HostingRepository	PRIDE
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-02-23_08:37:18.216.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ebtesam Gashash
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-23 01:10:08	ID requested
⏵ 1	2022-02-23 08:37:18	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Plasma von Willebrand Factore, vWF, occupancy, glycosylation, N-glycan, O-glycan, microheterogeneit, CID, HCD, mass spectrometry

Peng George Wang
contact affiliation	Chemistry Department Peng Wangs' Lab Georgia State University Atlanta, GA 30302 USA
contact email	pwang11@gsu.edu
lab head
Ebtesam Gashash
contact affiliation	Georgia State University
contact email	egashash1@student.gsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006567
     1. Label: PRIDE project
     2. Name: An Insight into Glyco-microheterogeneity of Plasma von Willebrand Factor (vWF) by Mass Spectrometry