PXD006564-1

PXD006564 is an original dataset announced via ProteomeXchange.

Title	DIAPH1 interaction partner identification using BioID.
Description	293T cells were transfected with p TRIPZ BirA tagged DIAPH1. Two days post transfection, cells were treated with puromycin to select transfectants. The entire BirA-DIAPH1 cassette is under the control of Tet inducible promoter. Induction of DIAPH1 was achieved by treating cells with Doxycycline (10ug/ml) for 24hrs followed by 50uM of Biotin treatment, overnight, to induce biotinylation of interacting partners. Cells treated with Biotin alone (no Dox) were used as a control for IP. Cytoplasmic extracts were prepared and subjected to immunoprecipitation using Streptavidin magnetic beads. Beads were washed with RIPA buffer and subjected to Mass spectrometry based protein identification to find interacting partners of DIAPH1.
HostingRepository	jPOST
AnnounceDate	2017-05-23
AnnouncementXML	Submission_2017-05-22_20:03:38.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gopinath M
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; N6-acetyl-L-lysine; (2S,3R)-3-hydroxyaspartic acid
Instrument	instrument: Orbitrap Fusion LC-MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-22 20:03:35	ID requested
⏵ 1	2017-05-22 20:03:39	announced
2	2018-09-19 00:13:22	announced	Updated CV, FTP location and Modification list.
3	2021-02-04 16:41:35	announced	2021-02-04: Updated FTP location.
4	2022-09-18 03:48:48	announced	2022-09-18: Updated FTP location.

Dataset with its publication pending

submitter keyword: DIAPH1-BioID

Dr. Prabha Sampath
lab head
Gopinath M
contact affiliation	Agency for Science Technology and Research
dataset submitter

jPOST dataset URI