PXD006558 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In-depth quantitative proteomics of signal pathways in BV2 microglial cell activation |
Description | Microglia become activated by disturbances in the homeostasis of their local microenvironment. While there are varying degrees of microglia activation, a pro-inflammatory reactive state induced by exposure to stimuli like LPS and IFN-γ. In this study, we identified a total of 5497 proteins in whole cell proteome and 4938 proteins for secretome of activated BV-2 mouse microglia cell line with LPS or IFN-γ using improved shotgun proteomic approach. From the differentially expressed proteins in stimulated microglia, we were able to classify pathways related immune-inflammatory response or metabolism. Moreover, we performed longitudinal quantification of secreted proteins during the detrimental activation of microglia which releases neurotoxic molecules mediated neuronal cell loss in the brain region. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:39:56.022.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jongmin Woo |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | 2x(13)C,6x(2)H labeled dimethylated L-arginine; acetylated residue; iodoacetamide derivatized amino-terminal residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-05-22 05:36:41 | ID requested | |
1 | 2017-08-09 01:25:26 | announced | |
⏵ 2 | 2024-10-22 04:39:56 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.jproteome.7b00445; |
Woo J, Han D, Wang JI, Park J, Kim H, Kim Y, Quantitative Proteomics Reveals Temporal Proteomic Changes in Signaling Pathways during BV2 Mouse Microglial Cell Activation. J Proteome Res, 16(9):3419-3432(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: dimethylation labeled quantification, quantitative analysis,BV2 activation |
Contact List
Youngsoo Kim |
contact affiliation | Departments of Biomedical Sciences, Seoul National University, Seoul, Korea |
contact email | biolab@snu.ac.kr |
lab head | |
Jongmin Woo |
contact affiliation | The University of North Carolina at Greensboro |
contact email | jongminw@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006558 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006558
- Label: PRIDE project
- Name: In-depth quantitative proteomics of signal pathways in BV2 microglial cell activation