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PXD006558-2

PXD006558 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn-depth quantitative proteomics of signal pathways in BV2 microglial cell activation
DescriptionMicroglia become activated by disturbances in the homeostasis of their local microenvironment. While there are varying degrees of microglia activation, a pro-inflammatory reactive state induced by exposure to stimuli like LPS and IFN-γ. In this study, we identified a total of 5497 proteins in whole cell proteome and 4938 proteins for secretome of activated BV-2 mouse microglia cell line with LPS or IFN-γ using improved shotgun proteomic approach. From the differentially expressed proteins in stimulated microglia, we were able to classify pathways related immune-inflammatory response or metabolism. Moreover, we performed longitudinal quantification of secreted proteins during the detrimental activation of microglia which releases neurotoxic molecules mediated neuronal cell loss in the brain region.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:39:56.022.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJongmin Woo
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList2x(13)C,6x(2)H labeled dimethylated L-arginine; acetylated residue; iodoacetamide derivatized amino-terminal residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-22 05:36:41ID requested
12017-08-09 01:25:26announced
22024-10-22 04:39:56announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.7b00445;
Woo J, Han D, Wang JI, Park J, Kim H, Kim Y, Quantitative Proteomics Reveals Temporal Proteomic Changes in Signaling Pathways during BV2 Mouse Microglial Cell Activation. J Proteome Res, 16(9):3419-3432(2017) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: dimethylation labeled quantification, quantitative analysis,BV2 activation
Contact List
Youngsoo Kim
contact affiliationDepartments of Biomedical Sciences, Seoul National University, Seoul, Korea
contact emailbiolab@snu.ac.kr
lab head
Jongmin Woo
contact affiliationThe University of North Carolina at Greensboro
contact emailjongminw@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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