PXD006558-2

PXD006558 is an original dataset announced via ProteomeXchange.

Title	In-depth quantitative proteomics of signal pathways in BV2 microglial cell activation
Description	Microglia become activated by disturbances in the homeostasis of their local microenvironment. While there are varying degrees of microglia activation, a pro-inflammatory reactive state induced by exposure to stimuli like LPS and IFN-γ. In this study, we identified a total of 5497 proteins in whole cell proteome and 4938 proteins for secretome of activated BV-2 mouse microglia cell line with LPS or IFN-γ using improved shotgun proteomic approach. From the differentially expressed proteins in stimulated microglia, we were able to classify pathways related immune-inflammatory response or metabolism. Moreover, we performed longitudinal quantification of secreted proteins during the detrimental activation of microglia which releases neurotoxic molecules mediated neuronal cell loss in the brain region.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:56.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jongmin Woo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2x(13)C,6x(2)H labeled dimethylated L-arginine; acetylated residue; iodoacetamide derivatized amino-terminal residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-22 05:36:41	ID requested
1	2017-08-09 01:25:26	announced
⏵ 2	2024-10-22 04:39:56	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00445;

Woo J, Han D, Wang JI, Park J, Kim H, Kim Y, Quantitative Proteomics Reveals Temporal Proteomic Changes in Signaling Pathways during BV2 Mouse Microglial Cell Activation. J Proteome Res, 16(9):3419-3432(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: dimethylation labeled quantification, quantitative analysis,BV2 activation

Youngsoo Kim
contact affiliation	Departments of Biomedical Sciences, Seoul National University, Seoul, Korea
contact email	biolab@snu.ac.kr
lab head
Jongmin Woo
contact affiliation	The University of North Carolina at Greensboro
contact email	jongminw@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006558

PRIDE project URI

• PRIDE
  1. PXD006558
     1. Label: PRIDE project
     2. Name: In-depth quantitative proteomics of signal pathways in BV2 microglial cell activation