PXD006556 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted proteomics of signal pathways in BV2 microglial cell activation |
Description | Microglia become activated by disturbances in the homeostasis of their local microenvironment. While there are varying degrees of microglia activation, a pro-inflammatory reactive state induced by exposure to stimuli like LPS and IFN-γ. In this study, using label-free PRM approach which is allowing us to measure the hyper-multiplex quantitative value of each protein with high-resolution mass spectrometry, we have quantified over 450 peptides at single run corresponding with the proteins belong to the pathways and directly or indirectly interacted proteins based on STRING database.Moreover, we performed longitudinal quantification of secreted proteins during the detrimental activation of microglia which releases neurotoxic molecules mediated neuronal cell loss in the brain region. |
HostingRepository | PRIDE |
AnnounceDate | 2017-08-09 |
AnnouncementXML | Submission_2017-08-09_01:01:57.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jongmin Woo |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; iodoacetamide derivatized amino-terminal residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-05-22 05:31:55 | ID requested | |
⏵ 1 | 2017-08-09 01:01:58 | announced | |
Publication List
Woo J, Han D, Wang JI, Park J, Kim H, Kim Y, Quantitative Proteomics Reveals Temporal Proteomic Changes in Signaling Pathways during BV2 Mouse Microglial Cell Activation. J Proteome Res, 16(9):3419-3432(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: BV2 activation, quantitative analysis, label-free PRM, Targeted protoemics |
Contact List
Youngsoo Kim |
contact affiliation | Departments of Biomedical Sciences, Seoul National University, Seoul, Korea |
contact email | biolab@snu.ac.kr |
lab head | |
Jongmin Woo |
contact affiliation | Seoul National University |
contact email | jongminw@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006556 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006556
- Label: PRIDE project
- Name: Targeted proteomics of signal pathways in BV2 microglial cell activation