PXD006513-1

PXD006513 is an original dataset announced via ProteomeXchange.

Title	A microsomal proteomics view of H2O2- and ABA-dependent responses
Description	The plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated NADPH oxidases. Although quantitative proteomics studies have been performed to identify ABA- or hydrogen peroxide (H2O2)-dependent proteins, little is known about the ABA- and H2O2-dependent microsomal proteome changes. Here, we examined the effect of 50 µM of either H2O2 or ABA on the Arabidopsis microsomal proteome using tandem mass spectrometry and identified 86 specifically H2O2-dependent and 52 specifically ABA-dependent proteins that are differentially expressed. We observed differential accumulation of proteins involved in the TCA cycle notably in response to H2O2. Of these, aconitase 3 responded to both H2O2 and ABA. Additionally, over 30 proteins linked to RNA biology responded significantly to both treatments. Gene ontology categories such as ‘response to stress’ and ‘transport’ were enriched, suggesting that H2O2 or ABA directly and/or indirectly cause complex and partly overlapping cellular responses.
HostingRepository	PRIDE
AnnounceDate	2017-08-22
AnnouncementXML	Submission_2017-08-22_07:08:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudius Marondedze
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-15 01:33:02	ID requested
⏵ 1	2017-08-22 07:08:05	announced
2	2024-10-22 04:37:55	announced	2024-10-22: Updated project metadata.

Alqurashi M, Thomas L, Gehring C, Marondedze C, - and ABA-Dependent Responses. Proteomes, 5(3):(2017) [pubmed]

curator keyword: Biological

submitter keyword: hydrogen peroxide (H2O2)

abscisic acid (ABA)

microsomal proteomics

quantitative proteomics

mass spectrometry

Christoph Gehring
contact affiliation	Department of Chemistry, Biology and Biotechnology, University of Perugia, Italy
contact email	c.a.gehring@molecular-signals.com
lab head
Claudius Marondedze
contact affiliation	University of Cambridge
contact email	cm833@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006513
     1. Label: PRIDE project
     2. Name: A microsomal proteomics view of H2O2- and ABA-dependent responses