PXD006474-2

PXD006474 is an original dataset announced via ProteomeXchange.

Title	Proteome analsyis of Translocation phenomenon
Description	Escherichia coli is the most widely used bacterial model organism and is the most commonly used host for the expression of recombinant proteins. Here we describe an unexpected protein translocation phenomenon in E. coli, whereby over-expression of recombinant proteins leads to high-level lysis-independent, mechanosensitive channel (MscL) dependent release of recombinant protein into the periplasmic space. Protein accumulation in the periplasm leads to protein release into the extracellular environment, independent of outer membrane vesicle formation. The translocated proteins retain their corresponding biological activity, and can be isolated directly from the extracellular medium with high purity and yield. Condition-specific metabolomic and proteomic analyses combined with statistical enrichment analysis indicate a role of both osmotic and translational stress responses in the regulation of the MscL-dependent translocation phenomenon. We suggest a model coupling translational stress to the regulation of MscL via the action of both osmotic stress and the Alternative ribosome-rescue factor A (ArfA) to explain this potentially very useful phenomenon.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:52.609.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006474
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Neil Dixon
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-09 23:45:31	ID requested
1	2018-03-02 00:37:55	announced
⏵ 2	2024-10-22 04:36:53	announced	2024-10-22: Updated project metadata.

10.1128/mbio.02118-17;

10.6019/PXD006474;

Morra R, Del Carratore F, Muhamadali H, Horga LG, Halliwell S, Goodacre R, Breitling R, Dixon N, Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins. mBio, 9(1):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Translocation, translatinla stress, MscL, ArfA

Neil Dixon
contact affiliation	MIB, Univesitry of Manchester
contact email	neil.dixon@manchester.ac.uk
lab head
Neil Dixon
contact affiliation	University of Manchester
contact email	neil.dixon@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD006474

PRIDE project URI

• PRIDE
  1. PXD006474
     1. Label: PRIDE project
     2. Name: Proteome analsyis of Translocation phenomenon