PXD006446-2

PXD006446 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of skeletal muscle reveals estrogen as a key regulator of muscle signaling in women
Description	Women’s aging is characterized by menopausal loss of ovarian function, which has been suggested as a contributing factor to aging-related muscle deterioration and predisposes to the metabolic dysfunctions. However, the underlying molecular mechanisms have remained unknown. To identify mechanisms, we utilized muscle samples from 24 pre- and postmenopausal women, established proteome-wide profiles and identified upstream regulators and downstream cellular pathways associated with the differences in age, menopausal status and use of hormone replacement therapy (HRT). None of the premenopausal women used hormonal medication while the postmenopausal women were monozygotic twin-sister pairs who were either current HRT users or had never used HRT. The proteomic analyses resulted in the quantification of 762 muscle proteins of which 158 were for the first time associated with female muscle aging. The Ingenuity Pathway Analysis pinpointed 17β-estradiol as a potential upstream regulator of the observed differences in the major downstream pathways including dysregulated cell death and glycolysis pathways. The results increase knowledge on the factors related to skeletal muscle signaling and aging. This is of importance, since the role of female sex hormones in the regulation of muscle cell signaling has been under appreciated and scarcely studied as compared to vast amount of data on male sex hormones and skeletal muscle. Our results clearly demonstrate the also female sex hormones and HRT should be considered as potential active players and an intervention targets to promote women’s muscular health.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:17.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maciej Lalowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-03 23:34:10	ID requested
1	2017-09-12 01:04:16	announced
⏵ 2	2024-10-22 04:36:25	announced	2024-10-22: Updated project metadata.

Laakkonen EK, Soliymani R, Karvinen S, Kaprio J, Kujala UM, Baumann M, Sipil, ä S, Kovanen V, Lalowski M, Estrogenic regulation of skeletal muscle proteome: a study of premenopausal women and postmenopausal MZ cotwins discordant for hormonal therapy. Aging Cell, 16(6):1276-1287(2017) [pubmed]

10.1111/acel.12661;

curator keyword: Biological, Biomedical

submitter keyword: Human, estrogen, HDMSE, aging, muscle, hormon replacement therapy

Marc H. Baumann
contact affiliation	Medicum,Faculty of Medicine Biochemistry/Developmental Biology Meilahti Clinical Proteomics Core Facility PO Box 63 (Haartmaninkatu 8) FI-00014 University of Helsinki Finland
contact email	marc.baumann@helsinki.fi
lab head
Maciej Lalowski
contact affiliation	University of Helsinki, Medicum, HiLIFE, Meilahti Clinical Proteomics Core Facility
contact email	maciej.lalowski@helsinki.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006446

PRIDE project URI

• PRIDE
  1. PXD006446
     1. Label: PRIDE project
     2. Name: Proteomic analysis of skeletal muscle reveals estrogen as a key regulator of muscle signaling in women