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PXD006426-2

PXD006426 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCowpox virus proteomics reveals massive ubiquitination and proteasomal degradation of viral proteins but stable virus cores
DescriptionThe ubiquitin-proteasome system (UPS) is essential for replication of Orthopoxviruses (OPV) like vaccinia and cowpox virus (CPXV). Although several proteome studies identified ubiquitin as part of OPV particles, distinct modification sites are largely unknown. Moreover, the UPS plays a key role in poxvirus core uncoating but the underlying mechanisms are still elusive. In the presented study we show that impairment of CPXV replication by proteasome inhibition is caused by a lack of uncoating which can be observed by electron microscopy. These results suggest, that UPS-dependent degradation of viral core proteins is the mechanism underlying CPXV genome uncoating. To proof this hypothesis we analyzed the mature virion ubiquitinome of CPXV using mass spectrometry (MS). We elucidated 137 conserved ubiquitination sites in 54 viral proteins among five CPXV strains verifying ubiquitin is a major poxvirus modification. Structural core proteins were massively ubiquitinated and virions contained large amounts of K48-linked polyubiquitin supporting the hypothesis. Hence, we aimed to show the proteasome-dependent degradation of CPXV core proteins in infected HeLa cells. However, using MS-based quantitative analysis of ubiquitinated virus proteins early in infection we were not able to show the degradation of viral core proteins. Instead, our results revealed the proteasomal degradation of viral proteins associated with the formation of prereplication sites early in infection.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:37:09.998.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarica Grossegesse
SpeciesList scientific name: Cowpox virus; NCBI TaxID: NCBITaxon:10243; scientific name: Cowpox virus (CPV); NCBI TaxID: 10243;
ModificationListubiquitination signature dipeptidyl lysine
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-02 05:44:43ID requested
12018-02-09 11:41:36announced
22024-10-22 04:37:13announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41598-018-20130-9;
Grossegesse M, Doellinger J, Fritsch A, Laue M, Piesker J, Schaade L, Nitsche A, Global ubiquitination analysis reveals extensive modification and proteasomal degradation of cowpox virus proteins, but preservation of viral cores. Sci Rep, 8(1):1807(2018) [pubmed]
Keyword List
submitter keyword: Ubiquitination, viral proteomics,Orthopoxvirus
Contact List
Andreas Nitsche
contact affiliationZBS1, Highly Pathogenic Viruses, Robert Koch-Institute, Germany
contact emailNitscheA@rki.de
lab head
Marica Grossegesse
contact affiliationRobert Koch Institute
contact emailgrossegessem@rki.de
dataset submitter
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