PXD006425 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In depth proteome profiling of Cumin by LTQ-Orbitrap Velos mass spectrometer |
Description | A shotgun proteomic approach was used to obtain comprehensive dataset of cumin proteins. For this purpose we applied a one dimensional electrophoresis coupled with high resolution mass spectrometry. The first step in our proteome analysis strategy, we evaluate different extraction methods, based on differential solubility of protein. Among those methods, Readyprep protein extraction kit provide high purity of protein extract, gave higher protein yield and typically good resolution of protein bands on one dimensional gel electrophoresis. Further nanoLC-MS/MS analysis by state-of-the-art linear ion trap (LTQ)-Orbitrap Velose mass spectrometer resulted in identification of very high number proteins. Bioinformatics analysis provide functional distribution of identified protein. To our knowledge, this is the first study aimed for in depth study of cumin proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:44.146.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Uzma Rana |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-05-02 05:44:12 | ID requested | |
1 | 2022-02-28 02:56:10 | announced | |
⏵ 2 | 2023-11-14 08:48:44 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Plant proteins, Cumin, LC-MS/MS |
Contact List
Uzma Zaman |
contact affiliation | Dow Research Institute of Biotechnology and Biomedical Sciences, Dow University of Health Sciences |
contact email | uzma.zaman@duhs.edu.pk |
lab head | |
Uzma Rana |
contact affiliation | Dow University of Health Sciences |
contact email | uzma.zaman@duhs.edu.pk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD006425 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006425
- Label: PRIDE project
- Name: In depth proteome profiling of Cumin by LTQ-Orbitrap Velos mass spectrometer