PXD006372 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress, PART 2 |
Description | Reactive oxygen species (ROS) are increasingly recognised as important regulators of cellular biology through the oxidative modification of protein cysteine residues. Comprehensive identification of redox-regulated proteins and cellular pathways are crucial to understand ROS-mediated events. Here, we present a new Stable Isotope Cysteine Labelling with IodoAcetamide (SICyLIA) MS-based proteomic workflow to assess protein cysteine oxidation in diverse cellular models and primary tissues. This approach informs on all possible cysteine oxidative modifications and achieves proteome-wide sensitivity with unprecedented depth without using enrichment steps. Our results suggest that acute and chronic oxidative stress cause metabolic adaptation through direct oxidation of metabolic and mitochondrial proteins. Analysis of chronically stressed fumarate hydratase-deficient mouse kidneys identified oxidation of proteins circulating in bio fluids, through which redox stress may affect whole-body physiology. Obtaining accurate peptide oxidation profiles from a complex organ using SICyLIA holds promise for future application to patient-derived samples in studies of human disease. PART 1 Hydrogen Peroxide PART 2 Primary immortalised kidney epithelial cells fumarate hydratase (FH) deficient PART 3 kidney tissues fumarate hydratase (FH) deficient |
HostingRepository | PRIDE |
AnnounceDate | 2018-03-06 |
AnnouncementXML | Submission_2018-03-06_04:18:20.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sergio Lilla |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | N-ethylmaleimide derivatized cysteine; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-25 01:37:07 | ID requested | |
⏵ 1 | 2018-03-06 04:18:21 | announced | |
2 | 2018-04-24 08:01:05 | announced | Updated publication reference for PubMed record(s): 29679077. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Oxidative stress, ROS, cysteine oxidation quantitation, Primary immortalised kidney epithelial cells fumarate hydratase (FH) deficient, Mouse kidney tissue fumarate hydratase (FH) deficient, High pH fractionation, Orbitrap HF, Proteome, Dimethyl labeling |
Contact List
Sara Rossana Zanivan |
contact affiliation | CRUK - Beatson Institute for Cancer Research - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom |
contact email | s.zanivan@beatson.gla.ac.uk |
lab head | |
Sergio Lilla |
contact affiliation | Beatson Institute for Cancer Research |
contact email | s.Lilla@beatson.gla.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006372
- Label: PRIDE project
- Name: Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress, PART 2