PXD006355-2
PXD006355 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein ID of ϕRP31 |
Description | Jumbo phages ϕRP31/ϕRP12 infecting Ralstonia solanacearum were isolated in Thailand. Virion proteins of ϕRP31 separated by SDS-PAGE were visualized with Coomassie Brilliant Blue. The protein bands were excised from the gel, digested with trypsin, and analyzed by liquid chromatography-tandem mass spectrometry (LTQ Orbitrap XL). Assignment of tandem mass spectrometry data to tryptic peptides encoded by phage open reading frames was completed using an established procedure (Ahmad et al., 2014). Based on these genomic similarities, proteomic tree and gene phylogenies, we propose that ϕRP12 and ϕRP31 are new members of the group of ϕKZ-related phages. |
HostingRepository | jPOST |
AnnounceDate | 2017-05-21 |
AnnouncementXML | Submission_2017-10-30_00:44:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miyako Nakano |
SpeciesList | scientific name: phage; |
ModificationList | iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid; unknown modification: Deamidated (N, Q) |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-04-23 23:43:12 | ID requested | |
1 | 2017-05-21 01:38:33 | announced | |
⏵ 2 | 2017-10-30 00:44:13 | announced | Updated PubMed. |
3 | 2018-09-19 00:12:31 | announced | Updated CV, FTP location and Modification list. |
4 | 2021-02-04 17:20:25 | announced | 2021-02-04: Updated FTP location. |
5 | 2022-09-18 02:19:01 | announced | 2022-09-18: Updated FTP location. |
Publication List
Matsui T, Yoshikawa G, Mihara T, Chatchawankanphanich O, Kawasaki T, Nakano M, Fujie M, Ogata H, Yamada T, Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase. Front Microbiol, 8():1010(2017) [pubmed] |
Keyword List
submitter keyword: jumbo phage, ϕRP31, ϕKZ-like phages, Ralstonia solanacearum |
Contact List
Miyako Nakano | |
---|---|
lab head | |
Miyako Nakano | |
contact affiliation | Hiroshima University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |