PXD006352 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cross-species complementation reveals conserved functions for EARLY FLOWERING 3 between monocots and dicots. |
Description | Plant responses to the environment are shaped by the external stimuli and internal signaling pathways. In both the model plant Arabidopsis thaliana and crop species, circadian clock factors have been identified as critical for growth, flowering and circadian rhythms. Outside of A. thaliana, however, little is known about the molecular function of clock genes. Therefore, we sought to compare the function of Brachypodium distachyon and Seteria viridis orthologs of EARLY FLOWERING3, a key clock gene in A. thaliana. To identify both cycling genes and putative ELF3 functional orthologs in S. viridis, a circadian RNA-seq dataset and online query tool (Diel Explorer) was generated as community resource to explore expression profiles of Setaria genes under constant conditions after photo- or thermo-entrainment. The function of ELF3 orthologs from A. thaliana, B. distachyon, and S. viridis were tested for complementation of an elf3 mutation in A. thaliana. Despite comparably low sequence identity versus AtELF3 (< 37%), both monocot orthologs were capable of rescuing hypocotyl elongation, flowering time and arrhythmic clock phenotypes. Molecular analysis using affinity purification and mass spectrometry to compare physical interactions also found that BdELF3 and SvELF3 could be integrated into similar complexes and networks as AtELF3, including forming a composite evening complex. Thus, we find that, despite 180 million years of separation, BdELF3 and SvELF3 can functionally complement loss of ELF3 at the molecular and physiological level. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-08 |
AnnouncementXML | Submission_2018-01-08_01:26:58.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD006352 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Bradley Evans |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | Phospho; Carboxymethyl; Deamidated; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-21 08:07:58 | ID requested | |
⏵ 1 | 2018-01-08 01:26:58 | announced | |
2 | 2018-05-23 01:56:18 | announced | Updated publication reference for DOI(s): 10.1002/pld3.18. |
3 | 2023-11-14 08:49:10 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Circadian Signaling, Arabidopsis, AP-MS, Orbitrap |
Contact List
Dmitri A. Nusinow |
contact affiliation | Donald Danforth Plant Scinece Center |
contact email | meter@danforthcenter.org |
lab head | |
Bradley Evans |
contact affiliation | Donald Danforth Plant Science Center |
contact email | bevans@danforthcenter.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006352
- Label: PRIDE project
- Name: Cross-species complementation reveals conserved functions for EARLY FLOWERING 3 between monocots and dicots.