PXD006335-2

PXD006335 is an original dataset announced via ProteomeXchange.

Title	SUMO targets the APC/C in a phosphorylation-dependent manner to regulate transition from metaphase to anaphase
Description	Signal transduction by Small Ubiquitin-like Modifier (SUMO) regulates a myriad of nuclear processes. Here, we report on the role of SUMO in mitosis in human cell lines. Knocking down the SUMO conjugation machinery resulted in a delay in mitosis and defects in mitotic chromosome separation. Searching for relevant SUMOylated proteins in mitosis, we identified the APC/C complex, a master regulator of metaphase to anaphase transition, as a SUMO target. The APC4 subunit is the major SUMO target in the complex, containing SUMO acceptor lysines at positions 772 and 798. SUMOylation increased APC/C ubiquitylation activity towards a subset of its targets, including the newly identified target KIF18B. Intriguingly, SUMOylation of APC/C was regulated by casein kinase driven phosphorylation of serines at positions 777 and 779. Combined, our findings demonstrate the importance of SUMO signal transduction for genome integrity during mitotic progression and reveal a triad of PTMs, cooperating to drive mitosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:38.415.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sabine Cuijpers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-19 06:44:02	ID requested
1	2018-09-19 08:15:54	announced
⏵ 2	2024-10-22 04:37:41	announced	2024-10-22: Updated project metadata.

Eifler K, Cuijpers SAG, Willemstein E, Raaijmakers JA, El Atmioui D, Ovaa H, Medema RH, Vertegaal ACO, SUMO targets the APC/C to regulate transition from metaphase to anaphase. Nat Commun, 9(1):1119(2018) [pubmed]

10.1038/s41467-018-03486-4;

curator keyword: Biological

submitter keyword: Anaphase promoting complex, APC4,SUMO, mitosis, phosphorylation, chromatin bridges, KIF18B, ubiquitin

Alfred C.O. Vertegaal
contact affiliation	Department of Molecular Cell Biology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
contact email	a.c.o.vertegaal@lumc.nl
lab head
Sabine Cuijpers
contact affiliation	Leiden University Medical Center
contact email	s.a.g.cuijpers@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD006335

PRIDE project URI

• PRIDE
  1. PXD006335
     1. Label: PRIDE project
     2. Name: SUMO targets the APC/C in a phosphorylation-dependent manner to regulate transition from metaphase to anaphase