PXD006329-2

PXD006329 is an original dataset announced via ProteomeXchange.

Title	Determination of the specificity profiling of native and synthetic cardosin B using proteomic identification of protease cleavage sites (PICS)
Description	We performed an analysis of the substrate specificity preferences of native and synthetic cardosin B using the Proteomic Identification of protease Cleavage Sites methodology and some differences regarding preference and stringency of the subsite binding pockets were observed. Results clearly suggest that synthetic cardosin B is more stringent than native cardosin B explaining the observed suitability of the synthetic protein as biotechnological tool in cheese industry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:32.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Cruz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	thioacylation of primary amines - site N-term; biotinylated residue; thioacylation of primary amines - site K; dimethylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-19 01:47:59	ID requested
1	2018-05-04 03:22:56	announced
⏵ 2	2024-10-22 04:37:33	announced	2024-10-22: Updated project metadata.

10.1007/s00253-017-8445-8;

Almeida CM, Manso JA, Figueiredo AC, Antunes L, Cruz R, Manadas B, Bur D, Pereira PJB, Faro C, Sim, õ, es I, Functional and structural characterization of synthetic cardosin B-derived rennet. Appl Microbiol Biotechnol, 101(18):6951-6968(2017) [pubmed]

curator keyword: Technical

submitter keyword: Cardosin, PICS

Isaura Simões
contact affiliation	CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal; Biocant, Biotechnology Innovation Center, 3060-197 Cantanhede, Portugal;
contact email	isimoes@biocant.pt
lab head
Rui Cruz
contact affiliation	Center for Neuroscience and Cell Biology
contact email	ruicruzcc@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD006329

PRIDE project URI

• PRIDE
  1. PXD006329
     1. Label: PRIDE project
     2. Name: Determination of the specificity profiling of native and synthetic cardosin B using proteomic identification of protease cleavage sites (PICS)